藻蓝蛋白对辐射致小鼠氧化损伤的保护作用  

Protective effect of phycocyanin against oxidative damage induced by radiation

作　　者：刘琪 李文军[2] 陆丽娜 谢园园 王明超 秦松[2] 杜振宁[1] 

机构地区：[1]烟台大学药学院,烟台264005 [2]中国科学院烟台海岸带研究所,烟台264003 [3]兰州大学核科学与技术学院,兰州730000

出　　处：《核技术》2018年第1期27-32,共6页Nuclear Techniques

基　　金：中国科学院战略性先导科技专项(No.XDA1102040300);海洋公益性行业科研专项(No.20120527);国家自然科学基金(No.41176144;No.41376139);国家科技支撑计划(No.2013BAB01B01);烟台市科技计划(No.2016JHZB007)资助;海洋经济创新发展区域示范专项海洋药源生物种质资源库建设项目(No.12PYY001SF08-ZKYHADS-1)~~

摘　　要：通过建立X射线辐射损伤小鼠模型,研究藻蓝蛋白(Phycocyanin,PC)对辐射引起鼠氧化损伤的保护作用。选取C57 BL/6雄性小鼠72只,按体重随机分为正常对照组、单纯辐射组、PC预防组、阳性对照组,每组18只。辐射前连续灌胃相应受试样品7 d,在第8 d,除正常组外,其余各组接受全身一次性6 Gy的X射线照射。各组分别在辐射后的1 d、3 d、7 d留取血浆和组织,并检测血浆及肝组织中的抗氧化酶活性及肝组织中活性氧自由基(Reactive Oxygen Species,ROS)含量。实验结果表明,藻蓝蛋白显著提高了辐射后小鼠血浆中和肝脏中抗氧化酶超氧化物歧化酶(Superoxide Dismutase,SOD)、谷胱甘肽过氧化物酶(Glutathione Peroxidase,GSH-PX)的活性(p<0.05),降低了丙二醛(Malondialdehyde,MDA)的含量(p<0.05),减少肝组织中ROS的含量(p<0.05)。结果表明,藻蓝蛋白可提高小鼠抗氧化能力,减轻辐射对机体造成的氧化损伤,对小鼠辐射损伤有较好的防护作用。[Background] Phycocyanin （PC） is a pigment protein present in cyanobacteria, cryptophyta, etc. [Purpose] This study aims at the protective effect of phycocyanin on oxidative damage induced by irradiation by establishing injury mouse model by X-ray irradiation. [Methods] 72 C57 BL/6 male mice were randomly divided into normal control group, irradiation model group, PC pretreatment group and positive control group. The mice were continuously fed for 7 d. On the 8th day of intragastric administration, except for the normal control group, the rest of the mice were subjected to total body irradiation to a dose of 6 Gy for the preparation of radiation damage model. The plasma and tissues were collected at 1 d, 3 d and 7 d after irradiation, and the activities of antioxidant enzymes in plasma and liver were measured. The content of reactive oxygen species （ROS） in liver tissue was detected by dihydroethidium （DHE） staining. [Results] Phycocyanin increased the activities of superoxide dismutase （SOD） and glutathione peroxidase （GSH-PX） in the plasma of mice after irradiation, and increased the activity of SOD （p〈0.05）, decreased malondialdehyde （MDA） content （p〈0.05）, and reduced liver tissue ROS content （p〈0.05）. [Conelusion] The results show that phycocyanin can improve the antioxidant capacity of mice, reduce oxidative damage to the body caused by irradiation, and provide a better protective effect on irradiation injury of mice.

关 键 词：藻蓝蛋白 X射线辐射 抗氧化系统 

分 类 号：TL7[核科学技术—辐射防护及环境保护] R963[医药卫生—微生物与生化药学]