关节软骨YKL-40表达水平与骨关节炎的相关性  

Association of articular cartilage YKL-40 levels with osteoarthritis

作　　者：关健[1] 谢磊[1] 丁罗宾[1] 薛伟[2] 王华军[3] 

机构地区：[1]石家庄市第三医院关节外科,石家庄050000 [2]邯郸市中心医院骨科,河北邯郸056000 [3]暨南大学第一临床医学院,暨南大学附属第一医院骨关节外科,广州510630

出　　处：《实用医学杂志》2018年第1期58-62,共5页The Journal of Practical Medicine

基　　金：河北省石家庄市科技支撑计划课题(编号：151460433A)

摘　　要：目的探讨骨关节炎关节软骨YKL-40表达水平与关节软骨病变程度的相关性。方法选择42例骨关节炎患者,采用X线Kellgren-Lawrence(K-L)分级标准将患者分为2级组、3级组和4级组。选取11例非膝骨关节炎患者作为正常对照组。采用免疫组织化学法检测关节软骨中YKL-40的表达水平。采用WOMAC评分系统评价各组患者膝关节病变程度。对比分析各组患者YKL-40表达水平,及其与膝骨关节炎严重程度的相关性。结果膝关节炎患者的关节软骨YKL-40表达水平高于非膝关节炎患者,各组膝骨关节炎患者关节软骨YKL-40表达水平差异有统计学意义,Ⅳ级患者高于Ⅲ级、Ⅱ级患者。关节软骨的YKL-40表达水平与WOMAC疼痛、僵硬、肢体功能以及总分呈正相关,与X线K-L分级呈正相关。结论关节软骨YKL-40表达水平与关节软骨破坏严重程度相关,可作为一种潜在的评估膝骨关节炎症状严重程度的生物标记物。Objective To determine the association of articular cartilage （AC） levels of YKL-40 with os- teoarthritis （OA） at different stages of symptomatic severity in patients with knee OA. Methods 42 patients with knee OA were recruited into this study. The radiographic disease severity of OA was assessed by the Kellgren-Law- rence （K-L） grading system. 11 patients with non knee OA were selected as normal control group. AC levels of YKL -40 were explored by immunohistochemical method. Symptomatic severity was determined using Western Ontario and McMaster Universities Arthritis Index （WOMAC） scores from OA patients. Results YKL-40 levels in AC were positively related to WOMAC pain, function scores and total scores. WOMAC stiffness scores did not correlat- ed with YKL-40 concentrations in AC. The correlation between AC YKL-40 levels and WOMAC scores was further analyzed by muhinomial logistic regression. Multiple regression analysis showed that the correlation between AC YKL-40 levels and WOMAC scores was still significant after adjusting for other confounding factors. AC YKL-40 levels were significantly correlated with K-L grading in patients with knee OA. Conclusions YKL-40 in AC can be used as a potential biomarker for assessing the symptomatic severity of OA.

关 键 词：骨关节炎 生物标记物 YKL-40 关节软骨 

分 类 号：R684.3[医药卫生—骨科学]