生物酶协同催化体系及其对羊毛纤维的作用机制  

Biological enzyme synergistic catalytic system and its mechanism on wool fiber

作　　者：刘建勇[1] 吴胜争 赵笑康 

机构地区：[1]天津工业大学纺织学院,天津300387

出　　处：《纺织学报》2018年第1期71-78,83,共9页Journal of Textile Research

基　　金：国家科技支撑计划项目(2014BAE01B02);科技型中小企业技术创新资金项目(13ZXCXSY08100)

摘　　要：为提高蛋白酶与羊毛的作用效果,减少羊毛损伤和缩短酶处理时间,通过研究活化剂存在条件下蛋白酶的催化有效性及活化剂与羊毛的反应速率,验证了由蛋白酶和活化剂组成的生物酶协同催化体系的可行性。并通过比较减量率、处理液中蛋白含量变化及纤维表面鳞片形态,证明在生物酶协同催化体系中活化剂、蛋白酶的相互协同作用可明显促进羊毛角蛋白的水解速度。经过对染色性能、氨基酸组成变化、阿尔瓦登反应及X射线光电子能谱分析表明:生物酶协同催化体系的作用机制是活化剂和蛋白酶相互协同作用,其中活化剂作用于二硫键,蛋白酶作用于酰胺键,从而快速有效地实现羊毛纤维的鳞片剥除。In order to improve the interaction effect of protease and wool, reduce the damage to wool and shorten the time of enzyme treatment, the catalytic effectiveness of protease in the presence of activator and the reaction rate between activator and wool were studied to verify the feasibility of biological enzyme synergistic catalysis system composed of protease and activator. The synergistic effect of protease and activator in the enzyme catalytic system was proved to promote the hydrolysis rate of wool keratin significantly, by comparing the reduction rate, the change in protein content in liquid and scale image. The analysis results of the dyeing properties, amino acid composition changes, Allworden reaction and X- ray photoelectron spectroscopy show that the mechanism of biological enzyme synergistic catalytic system is the synergistic effect of protease and activator. Activator acts on the disulfide bond and protease on the amide bond, resulting in the peeling of wool scales rapidly and effectively.

关 键 词：生物酶 协同催化 羊毛 鳞片 角蛋白 防缩处理 

分 类 号：TS195.5[轻工技术与工程—纺织化学与染整工程]