TNF-α对糖尿病肾病大鼠髓袢升支粗段管周膜KCNJ10(Kir4.1)的调控  

Regulation of TNF-α on Basolateral KCNJ10(Kir4.1) in the Thick Ascending Limb of Diabetic Nephropathy Rat

作　　者：孟庆民 赵日新 王伟群[1] 张明月[1] 郭思琪 隋洪玉[1] 

机构地区：[1]佳木斯大学基础医学院,黑龙江佳木斯154007

出　　处：《广东化工》2018年第4期39-40,共2页Guangdong Chemical Industry

基　　金：国家自然科学基金项目(编号:31400994);黑龙江大学生创新创业训练计划项目(编号:201610222030)

摘　　要：目的:探讨TNF-α对糖尿病肾病形成中髓袢升支粗段管周膜KCNJ10(Kir4.1)的调控。方法:取SD雄性大鼠随机分成4组,正常对照组(NC)、糖尿病模型组(DM)、NC+TNFR:Fc组和DM+TNFR:Fc组,20天后用Western blot方法检测髓袢升支粗段中TNF-α、KCNJ10、PLA2和PTK的蛋白表达情况。结果:DM组TNF-α蛋白表达显著增加;DM组KCNJ10蛋白表达降低,而PLA2和PTK蛋白表达增加;DM+TNFR:Fc组KCNJ10蛋白表达增加,而PLA2和PTK蛋白表达降低。结论:在糖尿病肾病形成中,TNF-α通过激活PLA2和PTK依赖途径抑制髓袢升支粗段管周膜KCNJ10(Kir4.1)的活性。Purpose： To investigate the regulation of TNF-α on basolateral KCNJ 10 （Kir4.1） in the thick ascending limb of diabetic nephropathy. Methods： The male Sprague-Dawley rats were randomly divided into 4 groups： normal control group （NC）, diabetic model group （DM）, NC ＋ TNFR：Fc group and DM ＋ TNFR：Fc group. The protein expression ofTNF-α, KCNJ10, PLA2 and PTK was detected by Western blot after 20 days. Result： The protein expression of TNF-a, PLA2 and PTK increased while KCNJ10 decreased in DM group. The protein expression of TNF-α, PLA2 and PTK decreased while KCNJ10 increased in DM ＋ TNFR：Fc group. Conclusion： In the formation of diabetic nephropathy, TNF-a inhibits the activity of basolateral KCNJ10 （Kir4.1） in the thick ascending limb by activating PLA2 and PTK-dependent pathways.

关 键 词：TNF—α糖尿病肾病 髓袢升支粗段 管周膜 KCNJ10(KiN.1) 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]