超高效液相色谱-串联质谱法测定人尿中8-羟基脱氧鸟苷的含量——对吸烟与结直肠癌相关性的探讨  

UHPLC-MS/MS Determination of 8-Hydroxy-2'-Deoxyguanosine in Human Urine——A Probe into the Correlation of Smoking with Colorectal Cancer

作　　者：谢聪 丁培丽 郭成 郑树[2] 曹小吉[1] 莫卫民[1] 

机构地区：[1]浙江工业大学化学工程学院,杭州310014 [2]浙江大学医学院附属第二医院肿瘤研究所恶性肿瘤预警与干预教育部重点实验室,杭州310009

出　　处：《理化检验（化学分册）》2018年第1期44-48,共5页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：浙江省科技厅分析测试项目(2015C37030);浙江省科技厅公益技术应用研究项目(2017C33014);中国博士后科学基金(2014M560474)

摘　　要：取经预处理的人尿(0.5 mL),加入同位素内标,经固相萃取柱纯化和真空离心干燥后用水0.2 mL溶解残渣。采用超高效液相色谱-串联质谱法测定DNA氧化损伤标志物8-羟基脱氧鸟苷(8-OHd G)的含量。按此法测定了65例正常人(吸烟及非吸烟者分别为21,44例)和61例直肠癌患者(吸收及非吸烟者分别为17,44例)尿液中8-OHd G的浓度水平。结果表明:吸烟正常人和吸烟结直肠癌患者的尿液8-OHd G浓度水平分别显著高于非吸烟正常人和非吸烟结直肠癌患者的;非吸烟结直肠癌患者和吸烟结直肠癌患者尿液8-OHd G浓度水平分别显著高于非吸烟正常人和吸烟正常人的。这表明吸烟作为一个风险因子,可能会促进DNA氧化损伤,进而诱导结直肠癌的发生。A portion （0. 5 mL） of pretreated human urine was taken and added with isotopic internal standard. It was purified by SPE and the eluate was centrifuged in vaccum to dryness. The residue was taken up with 0. 2 mL of water. 8-Hydroxy-2＇-deoxyguanosine （8-OHdG）, a symbolizing product of oxidation damaging of DNA, was determined by UHPLC-MS/MS. Urine samples were taken from 65 healthy （including 21 smokers and 44 non-smokers） and 61 patients suffering from colorectal cancer （including 17 smokers and 44 non-smokers）, and urinary 8-OHdG in these samples were determined by the proposed method. It was found that： ① concentration levels of urinary 8-OHdG for smokers （both of healthy persons and patients） were significantly higher than those for non-smokers; and ② concentration levels of urinary 8-OHdG for the patients （both non-smokers and smokers） were also significantly higher than the healthy persons （both smokers and non-smokers）. It was concluded that smoking, as a risk factor, promoted oxidative damage of DNA and led to occurrence of colorectal cancer.

关 键 词：超高效液相色谱-串联质谱法 吸烟 结直肠癌 8-羟基脱氧鸟苷 人尿 

分 类 号：O657.63[理学—分析化学]