γ-H2AX的表达对初诊多发性骨髓瘤患者的预后影响  

Influence of γ-H2AX expression on prognosis in patients with newly diagnosed multiple myeloma

作　　者：曹春 徐莉 冯娟 唐海龙 董红娟 顾宏涛 舒汨汨 白庆咸 梁蓉 张涛 杨岚 董宝侠 陈协群 高广勋 

机构地区：[1]第四军医大学第一附属医院血液内科/中国人民解放军血液病专病中心,西安710032

出　　处：《重庆医学》2018年第3期294-298,共5页Chongqing medicine

基　　金：国家自然科学基金资助项目(81172247);陕西省自然科学基础研究计划项目(2016JM8107;2017JM8025);陕西省社会发展科技攻关项目(2016SF-071)

摘　　要：目的探讨初诊多发性骨髓瘤(MM)患者骨髓中γ-H2AX的表达水平及其与患者预后的关系。方法选择该院初诊的MM患者为病例组,非造血系统肿瘤并经骨髓涂片和活检检查未见明显形态异常者为对照组。采用免疫组织化学染色检测病例组和对照组骨髓中γ-H2AX的表达水平,IPP半定量分析并比较其表达差异,并根据病例组γ-H2AX的表达量将其分为强表达和弱表达两组,探究γ-H2AX表达量与MM患者预后的关系。结果病例组患者骨髓中γ-H2AX表达显著高于对照组(P<0.05);且强表达组MM患者γ-H2AX的表达水平明显强于弱表达组(P<0.05)。结论γ-H2AX在MM患者的骨髓组织中表达水平增多,其强表达预示MM患者较短的生存时间。Objective To investigate the expresmon of bone marrow γ-H2AX in the patients with multiple myeloma（MM） and its eorrelation with the prognosis. Methods The patients with newly diagnosed MM in this hospital were selected as the case group, and the patients with non-hemopoietic system tumor without obvious morphological abnormalities by bone marrow smear and biopsy served as the control group. The immunohistoehemistry was adopted to detect the expression level of bone marrow γ-H2AX in the cases group and control group,the image-Pro Plus（IPP） semiquantitative analysis was performed. The expression differences were compared between the two groups,moreover the case group was re-divided into the strong expression group and weak expression group according to γ-H2AX expression level. Then the relation ship between γ-H2AX expression level and the prognosis in the patients with MM. Results The bone marrow γ-H2AX expression level in the case group was significantly higher than that in the control group （P〈0.05） ;the level of γ-H2AX expression in the strong expression group was significantly stronger than that in the weak expression group （P〈0.05）. Conclusion The level of γ-H2AX expression was higher among MM patients,and the over expression of γ-H2AX predicts the shorter survival time.

关 键 词：多发性骨髓瘤 DNA断裂 双链 免疫组织化学 预后 Γ-H2AX 

分 类 号：R551.3[医药卫生—血液循环系统疾病]