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机构地区:[1]首都医科大学中医约学院,中医络病研究北京市重点实验室,北京100069
出 处:《国际中医中药杂志》2018年第1期54-57,共4页International Journal of Traditional Chinese Medicine
基 金:基金项同:北京市属高校青年拔尖人才培育项目(CIT&TCD201404184)
摘 要:目的 建立珠子参药材中皂苷类成分HPLC特征图谱,为其质量控制提供依据。方法 采用HPLC法测定珠子参药材中皂苷类成分,色谱柱为Agilent Poroshell 120 C18(4.6 mm×100 mm, 2.7 μm),乙腈-0.1%磷酸水溶液作为流动相进行梯度洗脱,流速为1.0 ml/min,检测波长为203 nm,柱温为35 ℃。结果 通过对8批次珠子参药材中皂苷类成分HPLC特征图谱的研究,确定了14个共有峰,对照品比对指认了其中4个皂苷类成分,分别为人参皂苷Rb1、Ro、竹节参皂苷Ⅳa、人参皂苷Rd;8批珠子参药材中有7批相似度大于0.90。结论 该方法精密度高、重复性强、稳定性好,可用于珠子参药材的质量控制。Objective To establish HPLC characteristic chromatograms method for quality control of Saponins in Panacis Majoris Rhizoma. Methods The HPLC method was conducted based on a Agilent Poroshell 120 C18 (4.6 mm × 100 mm, 2.7 μm) column, with acetonitrile-0.1% orthophosphoric acid as mobile phase by gradient elution. The flow rate was 1.0 ml/min. The detection wavelength was at 203 nm and the column temperature was 35 ℃. Results Eight samples from different sources were analyzed by HPLC. Fourteen main marker peaks were selected in the Characteristic spectrum and four peaks of saponins were identified. The similarity of seven samples was over 0.9. Conclusions The precision, repeatability and stability method was standardized, and this method can be used for quality control of Panacis Majoris Rhizoma.
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