有氧运动对慢性应激大鼠空间学习记忆及海马齿状回BDNF表达的影响  

Effects of Aerobic Exercise on the Learning and Memory and Hippocampal Dentate Gyrus BDNF Expression in Chronic Stress Rats

作　　者：崔建梅[1] 杨文艳 药宏慧[2] 于芳[1] 薄媛媛 苏晓云[3] 庞立杰 

机构地区：[1]中北大学体育学院,山西太原030051 [2]太原学院体育系,山西太原030051 [3]山西医科大学汾阳学院,山西汾阳032200 [4]山西体育职业学院,山西太原030006

出　　处：《首都体育学院学报》2018年第1期78-82,87,共6页Journal of Capital University of Physical Education and Sports

基　　金：国家自然科学基金项目(30671019);山西省自然科学基金项目(2014011041-6)

摘　　要：通过研究跑台运动对慢性应激大鼠空间学习记忆能力及海马齿状回脑源性神经营养因子(BDNF)含量的改变,探讨有氧运动改善慢性应激大鼠认知能力的可能机制。方法:将健康成年Sprague-Dawley(8周)大鼠随机分为3组:正常对照组(C,n=10)、应激模型组(CUS,n=10)及应激运动组(CUS+E,n=10)3组,通过慢性不可预知应激建立大鼠抑郁模型。CUS+E组大鼠采取4周跑台运动,通过八臂迷宫实验观察跑台运动对应激大鼠学习记忆能力的影响;八臂迷宫实验结束后用ABC免疫组织染色法检测各组大鼠海马齿状回BDNF的分布。结果:与CUS组大鼠比较,CUS+E组大鼠八臂迷宫实验中大鼠完成八臂迷宫探索时间(P<0.05)及进入放饵料臂平均潜伏期均显著缩短(P<0.05),参考记忆(P<0.05)及总记忆错误次数(P<0.05)均显著增多;海马DG区BDNF数量(P<0.05)及面积(P<0.05)均显著增多。结论:当前研究发现4周跑台运动可能通过激活海马DG区BDNF通路,增强海马DG区BDNF的表达,改善慢性应激大鼠的学习记忆能力。Objective:To observe the effects of treadmill exercise on the learning,memory and Hippocampal DG(dentate gyrus) BDNF(brain derived neurotrophic factor) expression in CUS(chronic unpredictable mild stress)rats and explore the possible mechanism of regular aerobic exercise to improve learning and memory.Methods:30 adult SD(Sprague-Dawley) male rats were randomly assigned into 3 groups:control group(C),chronic unpredictable stress group rats(CUS) and stress exercise group(CUS+E).All rats were subjected to CUS except the control group.The learning and memory ability of rats were tested by radial arm maze test(CUS) after CUS+E group rats received 4-week treadmill exercise.Subsequently,ABC immunohistochemical staining detected BDNF expression in Hippocampus DG in every group rats.Results:Compared with the CUS group rats,the time of completing 8-arms radial maze and latency of entering into bait arms in ERM test significantly shortened(P<0.05,P<0.05),reference memory errors and total memory errors in RAM test significantly decreased(P<0.05,P<0.05); number and area of BDNF significantly increased in Hippocampal DG(P<0.05,P<0.05) in CUE+E group rats.Conclusion:The present experiment verified that 4-week treadmill exercise reverses stress-induced learning and memory impaired via an increase in BDNF signaling and may increase BDNF expression in Hippocampal DG.

关 键 词：跑台运动 学习记忆 海马DG 脑源性神经营养因子 

分 类 号：G804.2[文化科学—运动人体科学]