补肾养骨汤诱导多发性骨髓瘤细胞株KM3的凋亡及机制探讨  

Inductive effect of BuShenYangGuTang on apoptosis of multiple myeloma cell line KM3 and its mechanism

作　　者：方梓青 黄幸儒 陈旭 李志远 李振杰 张建国 方坚 

机构地区：[1]广州中医药大学第三临床医学院,510000 [2]广州市正骨医院 [3]广州市荔湾区骨伤科医院 [4]广州中医药大学第三附属医院

出　　处：《天津医药》2018年第1期7-11,共5页Tianjin Medical Journal

基　　金：广东省科技计划项目(2014A020212272);广东省中医药局重点项目(20164009)

摘　　要：目的探讨补肾养骨汤对人多发性骨髓瘤细胞株KM3细胞增殖、凋亡的影响及其机制。方法取对数生长期KM3细胞,分别加入5%、10%、20%的补肾养骨汤含药血清培养,培养12、24、48和72 h后,采用Cell CountingKit-8(CCK-8)法检测补肾养骨汤对KM3细胞增殖的影响;KM3细胞经过不同浓度含药血清培养72 h后,采用流式细胞术检测补肾养骨汤对KM3细胞周期和凋亡作用;RT-q PCR和Western blot检测B淋巴细胞瘤-2基因(Bcl-2)、Bcl-2相关蛋白(Bax)和核转录因子(NF)-κB的表达水平。结果补肾养骨汤抑制KM3细胞株的增殖,诱导KM3细胞株呈剂量依赖性凋亡,使KM3细胞周期停滞在G0/G1期,导致KM3细胞株Bcl-2、NF-κB表达下调,Bax表达上调。结论补肾养骨汤抑制KM3细胞的增殖,影响细胞周期,诱导其凋亡,此作用机制可能与Bcl-2、Bax和NF-κB表达异常有关。Objective To study the effects and mechanisms of Bu Shen Yang Gu Tang on proliferation and apoptosis ofmultiple myeloma cell line KM3.MethodsThe inhibitory effect of Bu Shen Yang Gu Tang on cell proliferation was assessedby CCK-8. Bu Shen Yang Gu Tang induced KM3 cell cycle arrest was analyzed by flow cytometry after propidium iodidestaining. Flow cytometry was also used to analyze the cell apoptosis after Annexin V-FITC staining. The expressions of Bcl-2, Bax and NF-κB were determined by RT-q PCR and Western blot assay.ResultsBu Shen Yang Gu Tang inhibited theKM3 cell proliferation in a dose-dependent manner. The expression levels of Bcl-2 and NF-κB were decreased, theexpression level of Bax was increased, and the cell cycle was arrested in G0/G1 phase after treatment withBu Shen Yang Gu Tang.ConclusionBu Shen Yang Gu Tang could inhibit the proliferation, arrest cell cycle and induce theapoptosis in KM3 cells, which may be related to the abnormal expressions of Bcl-2, Bax and NF-κB.

关 键 词：多发性骨髓瘤 细胞凋亡 细胞增殖 细胞周期 补肾养骨汤 KM3细胞 

分 类 号：R285.5[医药卫生—中药学]