HPLC法测定扶正平消胶囊中芍药苷的含量  

Determination of paeoniflorin in Chinese traditional herbal preparation Fuzhengpingxiao capsule using HPLC method

作　　者：付振贺 赵亮[1] 周瑾[1] 王新霞[1] 张国庆[1] 黄玉凤[1] FU Zhenhe;ZHAO Liang;ZHOU Jin;WANG Xinxia;ZHANG Guoqing;HUANG Yufeng(Department of Pharmacy , East-ern Hepatobiliary Surgery Hospital Affiliated to Second Military Medical University , Shanghai 200438 , Chin)

机构地区：[1]第二军医大学附属东方肝胆外科医院药材科,上海200438

出　　处：《药学实践杂志》2018年第1期68-70,79,共4页Journal of Pharmaceutical Practice

基　　金：军队医疗机构制剂单品种研究:扶正平消胶囊标准研究(14TG0700)

摘　　要：目的建立扶正平消胶囊中芍药苷含量的测定标准。方法采用HPLC法进行含量测定,色谱柱为Agilent Zorbax SB-C18柱(150mm×4.6mm,5μm),流动相:乙腈-5mmol/L磷酸二氢钠水溶液(10∶90),流速:1.0 ml/min,柱温:25℃;检测波长:230nm,进样量10μl,运行时间25 min。结果芍药苷在25.0~500.0μg/ml范围内线性关系良好(r=0.999 9),线性方程:Y=12.65 X+43.54,平均加样回收率为92.69%,RSD为1.77%。结论该方法操作易行、结果可靠、重复性好,可用于扶正平消胶囊中芍药苷的含量测定。Objective To determine the content of paeoniflorin in Fuzhengpingxiao capsule by HPLC method.MethodsThe content of paeoniflorin were determined by HPLC,and the column was an Agilent Zorbax SB-C18 column（150 mm×4.6 mm,5μm）;The mobile phase was acetonitrile and 5 mmol/L sodium dihydrogen phosphate（10∶90）,and the flow rate was set at 1 ml/min.Column temperature was 25°C,detection wavelength was 230 nm,injection volume was 10μl,and running time was 25 min.Results Paeoniflorin was linear（r=0.999 9）in the concentration of 25.0-500.0μg/ml,the linear equation was Y=12.65 X ＋43.54,and the average recovery was 92.69% with RSD value was 1.77%.Conclusion The method was easy to operate,reliable and reproducible,which could be used in the determination of paeoniflorin in FuzhengPingxiao capsule.

关 键 词：高效液相色谱 扶正平消胶囊 芍药苷 含量测定 

分 类 号：R284.1[医药卫生—中药学]