质粒DNA纯化及内毒素去除策略研究进展  

Progress of Purification of Plasmid DNA and Removal Strategy for Endotoxin

作　　者：李卫玲 易初丽 

机构地区：[1]江汉大学武汉生物医学研究院,湖北武汉430056

出　　处：《江汉大学学报（自然科学版）》2018年第1期62-66,共5页Journal of Jianghan University：Natural Science Edition

基　　金：3551光谷人才计划项目(全自动柱式全项核酸纯化仪器的研发及产业化)

摘　　要：质粒DNA(plasmid DNA)是最常用的基因工程和基因疫苗载体,近年来在生物医学和环境科学等领域得到了广泛应用。质粒DNA纯化是分子生物学研究中常用的实验技术,纳米磁性粒子等固相载体技术和色谱技术优化了DNA纯化方法。对大肠杆菌质粒DNA常用纯化方法、内毒素去除策略以及自动化纯化设备进行综述,为质粒DNA的应用研究提供支持。Plasmid DNA is most common carrier for genetic engineering and gene vaccine,it is widely applied in biomedical and environmental science in recent years. Purification of plasmid DNA is the common techndogy in molecular biology,while solid phase carriers such as magnetic nanoparticles and chromatographic techniques have promoted the methods of purification of DNA. We present the methodsof purification of plasmid DNA,endotoxin removal strategies and automatic nucleic acid purification device which are suited for plasmid DNA isolation,to privide the supports for application of plasmid DNA.

关 键 词：质粒DNA 内毒素 核酸自动纯化仪 

分 类 号：Q782[生物学—分子生物学]