薤白提取物对大鼠急性心肌缺血损伤的保护作用  

Protective Effect of Allium macrostemon Extract on Acute Myocardial Ischemia Injury in Rats

作　　者：雷杰 段刚峰[1] 

机构地区：[1]武汉市中西医结合医院心血管内科,湖北武汉430022

出　　处：《江汉大学学报（自然科学版）》2018年第1期67-71,共5页Journal of Jianghan University：Natural Science Edition

基　　金：武汉市卫生计生办科研项目(WZ14A06)

摘　　要：目的观察薤白提取物对大鼠急性心肌缺血损伤的保护作用,并探讨其作用机制。方法采用结扎左冠脉建立大鼠急性心肌缺血损伤模型,给予薤白提取物、硝酸甘油干预后,监测大鼠造模前后心电图Ⅱ导联ST段变化,检测血清谷胱甘肽转移酶(GSH-Px)、胆碱酯酶(TChE)的活性。游离脂肪酸(NEFA)、丙二醛(MDA)的水平;显微镜下观察心肌缺血损伤的病理学变化。结果与假手术组比较,模型组心电图ST段明显上移,血清GSH-Px活力明显减低(P<0.01),TCh E活力、NEFA、MDA含量明显升高(P<0.01),病理学研究提示心肌损伤较重;与模型组比较,药物组心电图ST段较低(P<0.01),血清GSH-Px活力明显增加(P<0.01),TCh E活力、NEFA、MDA含量明显降低(P<0.05,P<0.01),病理学研究发现心肌损害程度减轻。结论薤白提取物对大鼠急性心肌缺血损伤具有一定的保护作用。Objective To observe the protective effect of Allium macrostemon extract on acute myocardial ischemia injury in rats,and to explore its mechanism. Methods An acute myocardial ischemia injury model was established with ligation of the left coronary artery in rats,after intervention of Allium macrostemon extract and nitroglycerin,the changes of ST segment of ECG Ⅱ lead before and after mod-eling in rats were monitored;the levels of serum glutathione transferase(GSH-Px),cholinesterase(TCh E),nomesterified fatty acids(NEFA)and malondialdehyde(MDA)were measured;pathologi-cal changes of myocardial ischemia injury were observed under microscope. Results Compared with thesham operation group,the ST segment of ECG in the model group was obviously upshifted,the serumGSH-Px activity was significantly decreased( P < 0. 01),the activity of TCh E,the content of NEFAand MDA increased significantly( P < 0. 01),and the pathological study showed that the myocardial in-jury was more serious;compared with the model group,the ST segment of ECG was lower in the medicine groups( P < 0. 01),the serum GSH-Px activity was significantly increased in the medicine groups( P < 0. 01),the activity of TCh E and the content of NEFA and MDA were significantly decreased inthe medicine groups( P < 0. 05,P < 0. 01),the degree of myocardial damage reduced in pathological findings in the medicine groups. Conclusion Allium macrostemon extract has a protective effect on acute myocardial ischemia injury in rats.

关 键 词：薤白提取物 急性心肌缺血 谷胱甘肽转移酶 胆碱酯酶 游离脂肪酸 丙二醛 

分 类 号：R285.5[医药卫生—中药学]