NOS2基因单核苷酸多态性与单纯尘螨过敏性鼻炎相关性研究  

Association between single nucleotide polymorphisms in NOS2 gene and house dust mite-sensitive allergic rhinitis

作　　者：李景云[1] 张媛[1] 张罗[1,2] 

机构地区：[1]首都医科大学附属北京同仁医院耳鼻咽喉头颈外科北京市耳鼻咽喉科研究所教育部耳鼻咽喉头颈科学重点实验室鼻病研究北京市重点实验室,北京100730 [2]首都医科大学附属北京同仁医院鼻过敏科,北京100730

出　　处：《首都医科大学学报》2018年第1期51-56,共6页Journal of Capital Medical University

基　　金：国家自然科学基金重点国际合作项目(81420108009);国家自然科学基金重点项目(81630023);国家自然科学基金青年项目(81400447);国家自然科学基金面上项目(81570895);教育部长江学者和创新团队发展计划(IRT13082);北京市百千万人才工程资助项目(2016);北京市卫生系统高层次卫生技术人才(2009-2-007;2011-3-043;2014-3-018);北京市医院管理局使命人才计划(SML20150203);北京市科技项目统筹课题(Z171100000117002)~~

摘　　要：目的本研究探讨NOS2基因单核苷酸多态性(single nucleotide polymorphism,SNP)与单纯尘螨过敏性鼻炎(house dust mite-sensitive allergic rhinitis,HDM-sensitive AR)发生的相关性。方法研究募集了HDM-sensitive AR患者385例和健康对照受试者424例。采集外周静脉血,提取基因组DNA。Sequenom i PLEX Mass ARRAY质谱法对NOS2基因上的6个标签SNPs(rs2297516,rs7406657,rs3794764,rs2872753,rs2779249,rs3794766)进行基因分型。结果等位基因分析显示6个SNPs的等位基因频率在HDM-sensitive AR组与正常对照组间差异无统计学意义。基因型分析显示rs3794764的(AA+AG)基因型分布在HDM-sensitive AR患者组和正常对照组间差异存在统计学意义(P=0.047),相对GG基因型是HDM-sensitive AR的危险因素(OR=1.413,95%CI:1.005~1.987)。多元线性回归分析显示在健康对照人群中rs3794766基因型与血清总Ig E浓度具有显著相关性(P=0.019,β=-0.131,95%CI:-0.229^-0.021)。结论 NOS2基因可能是中国汉族人群HDM-sensitive AR的易感基因。Objective To investigate the association between single nucleotide polymorphisms（ SNP） in NOS2 gene and house dust mite-sensitive allergic rhinitis（ HDM-sensitive AR）. Methods A total of 385 HDM-sensitive AR patients and 424 control subjects were enrolled and genomic DNA was extracted from peripheral blood leucocytes of each participant. A total of 6 reprehensive marker SNPs in NOS2 were genotyped using Sequenom i PLEX Mass ARRAY platform. Results The allele frequency analysis did not show any significant associations between 6 SNPs in NOS2 and HDM-sensitive AR. The genotype analysis showed the significant difference in distribution of rs3794764_（ AA ＋ AG） between HDM-sensitive AR patients and controls（ P = 0. 047）. The rs3794764_（ AA ＋ AG vs GG） presented a risk among HDM-sensitive AR patients（ OR = 1. 413,95% CI： 1. 005-1. 987）. Multiple linear regression analysis indicated that only rs3794766 in NOS2 gene was significantly associated with total serum Ig E levels in control group（ P = 0. 019,β =-0. 131,95% CI：-0. 229--0. 021. Conclusion NOS2 genes variabilities might be associated with HDM-sensitive AR susceptibility in Han Chinese.

关 键 词：单纯尘螨过敏性鼻炎 一氧化氮合成酶2 单核苷酸多态性 关联分析 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]