下调SNHG16对胃癌细胞HGC-27细胞周期的影响  

Effects of gastric cancer cell lines HGC-27 cycles by knocdown SNHG16

作　　者：朱晓鸿 赵娟娟 刘娟娟 万颖 黄海 

机构地区：[1]遵义市第四人民医院,贵州遵义563000 [2]贵州医科大学医学检验学院临床生化教研室,贵州贵阳550004

出　　处：《贵州医药》2017年第12期1242-1244,共3页Guizhou Medical Journal

基　　金：国家自然科学基金[No.81460364和No.81760429]

摘　　要：目的下调长链非编码RNA SNHG16对人胃癌细胞HGC-27细胞周期的影响。方法实时荧光定量PCR(quantitative real time-PCR,qRT-PCR)法检测SNHG16siRNA在HGC-27细胞中的干扰效率;流式细胞术(FACS)检测敲低SNHG16后对HGC-27细胞周期的影响;蛋白免疫印迹法(Western blot)检测周期相关蛋白细胞周期素依赖性蛋白激酶2(CDK2)的改变情况。结果qRT-PCR检测转染SNHG16siRNA后,细胞HGC-27中SNHG16表达量明显下降;流式细胞术检测低表达SNHG16使细胞HGC-27细胞周期中S期被阻滞且CDK2蛋白相对表达量下降。结论敲低SNHG16后阻滞细胞HGC-27的S期以及CDK2蛋白的表达,从而抑制细胞生长。Objective To explore the expression of long non-coding RNA SNHG16 in human gastric cancer tissues and cell lines,and as well its clinical implication.Methods Quantitative real-time PCR（qRT-PCR）was performed to confirmed the low expression level of SNHG16 after the transfection of SNHG16 siRNA in cell line HGC-27.Knocdown SNHG 16 was detected to the effects of HGC-27 cell cycle by flow cytometry.The expression of cell cycle-related proteins CDK2 was analyzed by western blot（WB）.Results The qRT-PCR confirmed that expression of SNHG16 was significantly knockdown with the transfection of SNHG16 siRNA.Knockdown SNHG16 could suppress the cell HGC-27 in S phase and down-regulation the protein expression of CDK2.Conclusion SNHG16 may suppress the ability of cell HGC-27 proliferation through arresting cell cycle at s phase and decrease the expression of CDK2.

关 键 词：胃癌 长链非编码RNA SNHG16 实时荧光定量法 

分 类 号：R34[医药卫生—基础医学] R735