Autotoxicity of Phthalate Esters in Tobacco Root Exudates:Effects on Seed Germination and Seedling Growth  

作　　者：DENG Jiajun ZHANG Yanling HU Jiwei JIAO Jiaguo HU Feng LI Huixin ZHANG Shixiang 

机构地区：[1]Soil Ecology Laboratory,College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095(China) [2]~hengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001(China) [3]Guizhou Provincial Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment,Guizhou Nounal University,Guiyang 550001(China) [4]Testing Center of Agro-Product Quality and Safety of Jiangxi Province,Nanchang 330046(China) [5]Key Laboratory of Eco-Environmental and Tobacco Leaf Quality of CNTC,Zhengzhou 450001(China)

出　　处：《Pedosphere》2017年第6期1073-1082,共10页土壤圈（英文版）

基　　金：supported by the Key Laboratory Project of CNTC(No.110201603010);the Scientific and Technological Project of Zhengzhou Tobacco Research Institute of CNTC(No.112011CZ0580)

摘　　要：Autotoxicity is one of the major factors that impede continuous cropping.It is defined as the toxic influence of chemicals released from one plant species on the germination and growth of individuals of the same species.Here, in order to exam the autotoxicity of tobacco root exudates, root exudates were collected from tobacco plants grown both in cultural solution and on natural soil.Using ultraperformance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry, main autotoxic chemical substances in the root exudates were identified.The autotoxic effects of suspected autotoxins on seed germination(including germination rate,germination potential, germination index, and vigor index) and seedling growth were analyzed.Dibutyl phthalate(or diisobutyl phthalate), dioctyl phthalate, and diisooctyl phthalate were identified in tobacco root exudates.It was observed that high concentrations(greater than 0.5 mmol L^(-1)) of each identified phthalate ester caused significant(P < 0.05) inhibition of tobacco seed germination and seedling growth.It can be concluded that phthalate esters such as dibutyl phthalate, diisobutyl phthalate, and diisooctyl phthalate in tobacco root exudates may play an important role in tobacco autotoxicity.Autotoxicity is one of the major factors that impede continuous cropping.It is defined as the toxic influence of chemicals released from one plant species on the germination and growth of individuals of the same species.Here, in order to exam the autotoxicity of tobacco root exudates, root exudates were collected from tobacco plants grown both in cultural solution and on natural soil.Using ultraperformance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry, main autotoxic chemical substances in the root exudates were identified.The autotoxic effects of suspected autotoxins on seed germination（including germination rate,germination potential, germination index, and vigor index） and seedling growth were analyzed.Dibutyl phthalate（or diisobutyl phthalate）, dioctyl phthalate, and diisooctyl phthalate were identified in tobacco root exudates.It was observed that high concentrations（greater than 0.5 mmol L^（-1）） of each identified phthalate ester caused significant（P 〈 0.05） inhibition of tobacco seed germination and seedling growth.It can be concluded that phthalate esters such as dibutyl phthalate, diisobutyl phthalate, and diisooctyl phthalate in tobacco root exudates may play an important role in tobacco autotoxicity.

关 键 词：AUTOTOXINS germination index germination potential germination rate vigor index 

分 类 号：S572[农业科学—烟草工业]