裙带菜(Undaria pinnatifida)配子体培养条件的优化  

Optimization of the Gametophyte Cultivation Condition of UndariaPinnatifida

作　　者：徐群 刘岩[1] 徐静娟[1] 

机构地区：[1]中国海洋大学藻类学与藻类养殖研究室,山东青岛266003

出　　处：《海洋湖沼通报》2017年第5期102-108,共7页Transactions of Oceanology and Limnology

基　　金：国家海洋公益性行业科研专项(201305005)资助

摘　　要：以日本三陆地区的裙带菜(Undaria pinnatifida)配子体为实验材料,采用双因素完全交叉分组实验,研究了温度(18℃、21℃、24℃、27℃)、光照强度(50μE·m^(-1)·s^(-1)、80μE·m^(-1)·s^(-1)、110μE·m^(-1)·s^(-1)、140μE·m^(-1)·s^(-1))、培养方式(静置培养、磁力搅拌培养、充气悬浮培养)、接种密度(0.1g/L、0.2g/L、0.4g/L、0.6g/L、0.8g/L、1.0g/L)对裙带菜配子体生长的影响。结果显示,光强和温度等环境因素以及两者的交互作用对配子体生长有较大影响。21℃、50μE·m^(-1)·s^(-1)的条件组合是实验中配子体培养的最佳条件。在培养的第5d到第10d之间,裙带菜配子体克隆的细胞增长率最大,可在第10d对配子体进行继代培养,使其保持较高的增长率。培养方式和初始接种密度以及两者的交互作用对配子体生长有较大影响,初始接种密度为0.1~0.2g/L的充气培养条件是配子体培养的最佳条件组合。本实验得出,日本三陆裙带菜雌配子体克隆扩培的最适培养条件为:温度21℃、光强50μE·m^(-1)·s^(-1)、接种密度0.1~0.2g/L,充气悬浮培养。The main effects of different temperatures （18, 21, 24, and 27℃）, light intensities （50, 80,110 and 140μE·m^-1·s^-1）, cultivation methods （static culture, magnetic stirring culture, inflatable suspension culture）, and inoculation densities （0.1, 0.2, 0.4, 0.6, 0.8, and 1.0g/L） on the growth of Undaria gametophytes were studied under laboratory conditions. The results showed that the light intensity and the temperature as well as their interaction have great impacts on the gametophyte growth. The studies revealed that the optimum environmental factors for the growth of U. pinnatifida were 50 E m^-1·s^-1 and 21℃. From the 5th to the 10th day, the U. pinnatifida gametophytegrowth rate was significantly higher than that on other days, and consequently to maintain a high growth rate, the 10th day culture was chosen for subculture. The cultivation method and the inoculation density as well as their interaction had a great impact on the growth of gametophytes. The results showed thatthe opti-mum factors for the growth of U. pinnatifida were 0.1-0. 2g/L and inflatable suspension for the ini- tial inoculation density andas the cultivation method,respectively. Generally speaking, U. pinnatifida grows well underthe following conditions ： temperature 21℃, light intensity50μE·m^-1·s^-1, inoculation density 0.1-0. 2g/L and inflatable suspension cultivation.

关 键 词：裙带菜 配子体 培养条件 

分 类 号：S946.1[农业科学—水产养殖]