雾化吸入及气管滴入阿米卡星、替加环素对健康小鼠支气管和肺组织的损伤  

Damage of bronchus and lung tissue in healthy mice after aerosol inhalation and intratracheal administration of amikacin and tigecycline

作　　者：郑亮[1] 亓倩[1] 周玉法[1] 李玉[2] 

机构地区：[1]莱芜市人民医院呼吸内科,山东莱芜271100 [2]山东大学齐鲁医院呼吸内科,山东济南250012

出　　处：《山东大学学报（医学版）》2017年第12期24-30,35,共8页Journal of Shandong University:Health Sciences

基　　金：山东省科学技术发展计划(2012GSF11859)

摘　　要：目的探讨雾化吸入及气管滴入阿米卡星、替加环素对健康小鼠支气管和肺组织损伤的不同。方法将144只昆明种小白鼠随机分为9组(每组16只):空白对照组、生理盐水吸入组、阿米卡星低浓度吸入组、阿米卡星高浓度吸入组、替加环素低浓度吸入组、替加环素高浓度吸入组、生理盐水滴入组、阿米卡星滴入组及替加环素滴入组,各组给予相应处理,取各组小鼠支气管和肺组织两处的病理标本,比较各组小鼠支气管和肺泡损伤程度的不同。结果替加环素高浓度吸入组及阿米卡星高浓度吸入组小鼠的肺损伤程度评分均显著高于空白对照组(P=0.001;P<0.001)。阿米卡星高浓度吸入组小鼠的肺损伤程度评分显著高于生理盐水滴入组(P<0.001),亦显著高于替加环素滴入组(P=0.001)。结论无论雾化吸入还是气管内滴入均对小鼠肺组织有损伤,而雾化吸入阿米卡星对小鼠肺泡损伤程度高于气管滴入替加环素。Objective To compare bronchus and lung tissue damage of healthy mice after aerosol inhalation and intratracheal administration of amikacin and tigecycline. Methods One hundred and forty-four Kunming species of mice were randomly divided into 9 groups（ 16 mice per group） ： blank control group,normal saline inhalation group,amikacin lowconcentration inhalation group,amikacin high concentration inhalation group,tigecycline lowconcentration inhalation group,tigecycline high concentration inhalation group,normal saline intratracheal administration group,amikacin intratracheal administration group and tigecycline intratracheal administration group. Each group was given the corresponding treatment. Then bronchus and lung tissue pathological specimens of each group of mice were obtained. The bronchus and lung tissue damage was compared among each group. Results Alveolar damage scores of tigecycline high concentration inhalation group and amikacin high concentration inhalation group were significantly higher than that of blank control group（ P = 0. 001; P〈0. 001）. Alveolar damage scores of amikacin high concentration inhalation group was significantly higher than that of normal saline intratracheal administration group（ P〈0. 001） and tigecycline intratracheal administration group（ P = 0. 001）. Conclusion Alveolar might be damaged by aerosol inhalation or intratrachealadministration of amikacin and tigecycline. The lung tissue damage after aerosol inhalation of amikacin might be more serious than intratracheal administration of tigecycline.

关 键 词：雾化吸入 气管滴注 阿米卡星 替加环素 支气管 肺组织 损伤 

分 类 号：R37[医药卫生—病原生物学]