Application of DNA Barcode Technology to the Identification of Exotic Mosquitoes at Ningbo Port  

应用DNA条形码技术鉴定宁波口岸入境蚊类（英文）

作　　者：吴薇[1] 王刚 徐翮飞[3] 赵彤言[4] 夏才军 

机构地区：[1]宁波出入境检验检疫局,浙江宁波315800 [2]浙江出入境检验检疫局,浙江杭州310016 [3]山东出入境检验检疫局,山东青岛266071 [4]军事医学科学院微生物流行病研究所,病原微生物生物安全国家家重点实验室,北京100071

出　　处：《Agricultural Science & Technology》2017年第12期2454-2457,共4页农业科学与技术（英文版）

摘　　要：[Objective] The study aimed to solve the problem of morphological identi- fication difficulties and propose solutions for the identification of foreign mosquitoes that are difficult to identify. [Method] Based on the sequencing, alignment and anal- ysis of COl gene fragment, DNA barcode technology was used to identify 7 exotic mosquitoes, and the phylogenetic analysis was performed using MEGA6.0 and DNASTAR software. Then the morphological characteristics of the mosquitoes were reviewed. [Result[ These mosquitoes were Armigeres subalbatus, Culex gefidus, Anopheles gambiae, and Culiseta incidens. [Conclusion] DNA barcode technology is a useful supplement to the morphological classification method of mosquitoes.[目的]解决形态学鉴定困难的问题,为进一步开展外来疑难蚊类的鉴定提出了解决方案。[方法]应用DNA条形码技术对宁波口岸入境蚊类的COⅠ基因片段进行了序列测定,用MEGA6.0和DNASTAR软件进行比对和分析,同时进行形态学特征复核。[结果]截获的7只蚊虫分别为骚扰阿蚊(Armigeres subalbatus)、白雪库蚊(Culex gelidus)、冈比亚按蚊(Anopheles gambiae)、incidens脉毛蚊(Culicella incidens)。[结论]DNA条形码技术可作为蚊类形态分类方法的有益补充。

关 键 词：Mosquito： DNA barcode ENTRY 

分 类 号：R185[医药卫生—流行病学]