PtrDREB28转录因子的克隆及抗逆性  

Cloning and Stress Resistance Analysis of PtrDREB28 Transcription Factors

作　　者：刘海妹 陈韵琳[1] 李成浩[1] 

机构地区：[1]林木遗传育种国家重点实验室(东北林业大学),哈尔滨150040

出　　处：《东北林业大学学报》2018年第2期12-16,39,共6页Journal of Northeast Forestry University

基　　金：国家"863"计划重点项目(2013AA102704)

摘　　要：DREB/CBF转录因子响应植物干旱、高盐和低温等非生物胁迫应答是通过特异结合DRE/CRT顺式作用元件。利用RT-PCR技术从毛果杨克隆PtrDREB28转录因子基因,用基因枪法对其进行亚细胞定位分析,结果表明其定位在细胞核中;利用农杆菌介导法对野生型大青杨进行遗传转化,并对转基因株系进行抗逆性分析,结果显示经高盐胁迫处理的转基因株系的耐受性明显高于野生型植株;通过测定和比较经盐胁迫处理后转基因株系和野生型植株的丙二醛质量摩尔浓度和相对电导率,发现转基因株系的丙二醛质量摩尔浓度和相对电导率均显著低于野生型植株,表明PtrDREB28基因的过表达可以显著提高杨树耐盐性,由此推测PtrDREB28转录因子可能参与杨树逆境胁迫应答过程。DREB/CBF transcription factors can respond to abiotic stresses, such as drought, high salinity and low temperature, through specifically combined with DRE/CRT cis acting elements. A PtrDREB28 transcription factor gene was cloned from Populus trichocarpa by RT-PCR. The analysis of subcellular localization by particle bombardment showed that PtrDREB28 was in the nucleus. To conduct genetic transformation in P. ussurien,sis by agrobacterium mediated genetic transformation and the transgenic plants were treated with stress resistance analysis to text resistance. The tolerance of transgenic plants treated with high salt stress was significantly higher than that of wild type. Through the determination and comparison with the content of malondialdehyde and relative conductivity of transgenic plants and wild type plants after salt stress treatment, the content of malondialdehyde and relative conductivity of transgenic plants were significantly lower than that of wild plant, and it is inferred that the functions of PtrDREB28 transcription factor may be related to stress responses in Pooulus.

关 键 词：PtrDREB28基因 转录因子 大青杨 转基因 非生物胁迫 

分 类 号：S722.36[农业科学—林木遗传育种]