4株H5N6亚型禽流感病毒毒株的分离鉴定与致病性研究  

Isolation,Identification and Pathogenicity Analysis of Four H5N6 Avian Influenza Virus Isolates

作　　者：周佩娇 邢利 贾伟新 廖明 

机构地区：[1]华南农业大学兽医学院,人兽共患病防控制剂国家地方联合工程实验室,农业部人畜共患病重点实验室,农业部兽用疫苗创制重点实验室,广东省动物源性人兽共患病预防与控制重点实验室,广州510640 [2]广州市南沙区动物防疫监督所,广州511458

出　　处：《畜牧兽医学报》2018年第1期147-156,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：科技基础性工作专项(2012FY111000);现代农业产业技术体系建设专项资金(CARS-42-G09);农业部动物疫情监测与防治项目(农医发[2016]11号);东莞市科技计划项目(2014108101041)

摘　　要：本研究旨在研究4株H5N6亚型禽流感病毒分离株的生物学特性与对鸡的致病性。对2015—2016年广东地区的临床送检样品进行病毒分离鉴定,对分离株进行基因克隆、测序和序列分析,并对4周龄SPF鸡点眼、滴鼻攻毒。结果分离得到4株H5N6亚型禽流感病毒,其HA基因属于Clade2.3.4.4,HA蛋白的裂解位点处具有多个连续碱性氨基酸,具备高致病性禽流感病毒的分子特征。攻毒组鸡5d内全部死亡,同居组9d内全部死亡;攻毒后鸡持续排毒,在心、肝、脾、肺、肾、脑等组织脏器中病毒滴度高,且对组织造成广泛性损伤。4株所测试的H5N6亚型禽流感病毒分离株对鸡具有高致病性和水平传播能力,提示需加强H5N6亚型禽流感的防控。The purpose of this study was to investigate the biological characteristics of 4 H5N6 subtype avian influenza virus isolates, and their pathogenicity to chickens. In this study, the clinical samples between 2015-2016 year in Guangdong were used to isolate virus, and the isolates were identified, the genes of isolates were cloned, sequenced and analyzed, then 4 weeks old SPF chickens were infected by the way of intranasal and eyedrop inoculation. Our results showed that 4 strains of H5N6 avian influenza virus were isolated, their HA gene belonged to Clade 2.3.4.4, and the isolates contained multiple basic amino acids at the cleavage site, which were characteristic of highly pathogenic AIV. The inoculation results showed that all chickens of the attack group died in 5 d, and the chickens of cohabitation group died in 9 d; the infected chickens could shed virus continuously. And the avian influenza virus could be detected in the heart, liver, spleen, lung, kidney, brain in high titer and caused extensive pathological damage. The 4 strains of H5N6 subtype avian influenza virus isolates showed high pathogenicity and horizontal transmission ability to chickens, suggesting the need to strengthen the prevention and control of H5N6 subtype avian influenza.

关 键 词：H5N6 禽流感病毒 SPF鸡 致病性 

分 类 号：S852.659.5[农业科学—基础兽医学]