禽网状内皮组织增生病病毒感染SPF雏鸡血液淋巴细胞增殖功能及其细胞周期相关因子变化  

Changes of Lymphocytes Proliferative Function and Cell Cycle Related Factors Expression in Peripheral Blood of SPF Chicks Infected with Reticuloendotheliosis Virus

作　　者：王晓燕 刘文超[1] 付礼胜 高雪丽[1] 刘超男[1] 吕晓萍[1] 郑世民[1] 

机构地区：[1]东北农业大学动物医学学院黑龙江省实验动物与比较医学重点实验室,哈尔滨150030

出　　处：《畜牧兽医学报》2018年第1期157-163,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31472169)

摘　　要：为探索禽网状内皮组织增生病病毒(REV)感染对SPF雏鸡外周血液的淋巴细胞增殖功能及其细胞周期素D1、p27表达的影响。将80只1日龄SPF雏鸡随机分为REV感染组和对照组,应用CCK-8、RT-qPCR、Western Blot等方法对雏鸡外周血液上述被检指标的动态变化进行检测。结果表明,REV感染SPF雏鸡后,其外周血液中T、B淋巴细胞增殖能力明显低于对照组雏鸡,且p27mRNA水平显著(P<0.05)高于对照组雏鸡;病毒感染后期Cyclin D1mRNA表达及其蛋白质含量均显著高于(P<0.05)对照组雏鸡,然而p27转录显著低于对照组雏鸡(P<0.05)。REV感染SPF雏鸡导致的血液T、B细胞增殖能力下降、Cyclin D1mRNA转录及其蛋白质含量的增加而p27mRNA转录下降与REV感染所致的雏鸡免疫机能下降密切相关。This experiment was conducted to explore the proliferation function of T, B lymphocytes and expression of Cyclin D1 and p27 in peripheral blood of SPF chicks infected with reticuloendotheliosis virus （REV）. Eighty 1-day-old SPF chicks were randomly divided into REV infection group and control group. The T, B lymphocytes proliferation function and expression of Cyclin D1 at mRNA and protein level, transcription of p27 in peripheral blood were detected by CCK-8, RT-qPCR and Western Blot. The results showed that the proliferation ability of T and B lymphocytes in peripheral blood of SPF chicks infected with REV early was significantly lower than that of the control chicks, and the transcription of p27 mRNA was significantly higher than that of the control chicks. The expression of Cyclin D1 mRNA and protein content in the late stage of infection were significantly higher than that of the control chicks （P〈0.05）, but the transcription of p27 mRNA were significantly lower than those of control chicks （P〈0.05）. The above results showed that the decrease of T, B lymphocytes proliferative function and p27 mRNA transcription, and increase of Cyclin D1 at mRNA and protein level in blood of SPF chicks were closely related to the decrease in immune function of chicks infected with REV.

关 键 词：SPF雏鸡 禽网状内皮组织增生病病毒 细胞周期素D1 淋巴细胞增殖能力 外周血液 

分 类 号：S852.659.3[农业科学—基础兽医学]