GLYX-13对小鼠长时间异氟醚麻醉后认知功能的影响  

Effect of GLYX-13 on cognitive function after long-time isoflurane anesthesia in mice

作　　者：刘欢 许芳霞[2] 崔银[2] 夏天娇[2] 张朋[2] 顾小萍[1] 马正良[1] 

机构地区：[1]南京医科大学鼓楼临床医学院,210008 [2]南京大学医学院附属鼓楼医院麻醉科,210008

出　　处：《中华麻醉学杂志》2017年第10期1196-1200,共5页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81371207）

摘　　要：目的 评价GLYX-13对小鼠长时间异氟醚麻醉后认知功能的影响.方法 健康雄性C57∕B6J小鼠192只,8周龄,体重22～25 g,采用随机数字表法分成4组（n=48）：对照组（C组）、异氟醚麻醉组（I组）、GLYX-13组（G组）和异氟醚麻醉 ＋ GLYX-13组（IG组）.I组和IG组接受1.5%异氟醚麻醉6 h.G组和IG组于麻醉前2 h尾静脉注射GLYX-131 mg∕kg.于麻醉后第1、3和7天时进行新物体识别实验和条件性恐惧记忆实验.于麻醉后第1、3和7天行为学测定结束后,取8只小鼠,采用 Q-PCR 法测定海马含2B 亚基的 NMDA 受体（NR2B）和环磷腺苷效应元件结合蛋白（CREB）的mRNA表达水平.结果 与C组比较,I组新物体探索时间百分比、辨别指数和僵直时间百分比降低,海马NR2B mRNA和CREB mRNA表达下调（P〈0.05）.与I组比较,IG组新物体探索时间百分比、辨别指数和僵直时间百分比升高,海马 NR2B mRNA 和 CREB mRNA 表达上调（P〈0.05）.结论 GLYX-13可显著改善小鼠长时间异氟醚麻醉后认知功能.Objective To evaluate the effects of GLYX-13 on cognitive function after long-time isoflurane anesthesia in mice. Methods A total of 192 healthy male C57∕B6J mice, aged 8 weeks, weig-hing 22-25 g, were divided into 4 groups（n=48 each）using a random number table： control group （group C）, isoflurane anesthesia group（group I）, GLYX-13 group（group G）, and isoflurane anesthesia plus GLYX-13 group（group IG）. The animals were exposed to 15% isoflurane for 6 h in I and IG groups. GLYX-13 1 mg∕kg was injected via the caudal vein at 2 h before anesthesia in G and IG groups. Novel ob-ject recognition test and contextual fear conditioning test were performed on 1st, 3rd and 7th days after an-esthesia. The expression of 2B subunits-containing NMDA receptor（NR2B）and cyclic adenosine mono-phosphate response element-binding protein（CREB）mRNA in the hippocampus was detected by quantita-tive real-time polymerase chain reaction after the end of behavioral tests on 1st, 3rd and 7th days after anes-thesia. Results Compared with group C, the percentage of time spent in exploring a novel object, dis-crimination index and percentage of freezing time were significantly decreased, and the expression of NR2B and CREB mRNA in the hippocampus was down-regulated in group I（P 〈0.05）. Compared with group I, the percentage of time spent in exploring a novel object, discrimination index and percentage of freezing time were significantly increased, and the expression of NR2B and CREB mRNA in the hippocampus was up-regulated in group IG（P 〈0.05）. Conclusion GLYX-13 can significantly improve the cognitive func-tion after long-time isoflurane anesthesia in mice.

关 键 词：兴奋性氨基酸激动剂 异氟醚 认知障碍 

分 类 号：R614[医药卫生—麻醉学]