舒芬太尼对小鼠周围神经损伤后神经组织碱性成纤维细胞生长因子表达的影响  

Effect of sufentanil on expression of basic fibroblast growth factor in nerve tissues after peripheral nerve injury in mice

作　　者：孙义[1] 张析哲[1] 周琪[1] 曹剑[2] 梁晓东[1] 宋健楠[1] 毕立伟 李海波[1] 

机构地区：[1]内蒙古赤峰市医院麻醉科,024000 [2]内蒙古赤峰市医院骨科,024000

出　　处：《中华麻醉学杂志》2017年第10期1250-1253,共4页Chinese Journal of Anesthesiology

摘　　要：目的 评价舒芬太尼对小鼠周围神经损伤后神经组织碱性成纤维细胞生长因子（bF-GF）表达的影响.方法 清洁级健康雄性Balb∕c小鼠100只,6～8周龄,体重18～22 g,采用随机数字表法分为4组（n=25）：周围神经损伤组（PNI组）、舒芬太尼高剂量组（H组）、舒芬太尼中剂量组（M组）和舒芬太尼低剂量组（L组）.制备单侧坐骨神经离断损伤模型后,于造模后即刻 H组、M组、L组分别腹腔注射舒芬太尼10.0、5.0和2.5 μg∕kg,1次∕d,连续给3 d.PNI组注射等容量生理盐水.于造模后1、2、4、8、12周时,取5只小鼠处死,取损伤处上下0.5 cm的神经组织,电镜下观察神经纤维的髓鞘形态.采用Western blot法测定坐骨神经bFGF的表达.结果 PNI组髓鞘形状不规则,板层厚度较薄,分离严重,脱髓鞘改变,雪旺细胞增殖不明显;H组髓鞘形状规则,髓鞘板层致密,雪旺细胞增殖明显;M组髓鞘形状大部分规则,板层较薄,有雪旺细胞增殖;L组髓鞘形状不规则,板层分离,脱髓鞘改变,雪旺细胞增殖不明显.与PNI组比较,H组、M组和L组坐骨神经bFGF表达上调（P〈0.05）;与L组比较,H组和M组坐骨神经bFGF表达上调（P〈0.05）;与M组比较,H组坐骨神经bFGF表达上调（P〈0.05）.结论 舒芬太尼促进小鼠周围神经损伤后再生和修复的机制可能与上调神经组织bFGF表达有关.Objective To evaluate the effect of sufentanil on the expression of basic fibroblast growth factor（bFGF）in nerve tissues after peripheral nerve injury in mice. Methods One hundred pathogen-free healthy male Balb∕c mice, aged 6-8 weeks, weighing 18-22 g, were divided into 4 groups （n=25 each）using a random number table： peripherial nerve injury group（group PNI）, high dose sufentanil group（group H）, medium dose sufentanil group（group M）and low dose sufentanil group （group L）. The model of unilateral sciatic nerve transaction was established in ketamine-anesthetized mice. Sufentanil 100, 50 and 25 μg∕kg were intraperitoneally injected immediately after establishment of the model once a day for 3 consecutive days in H, M and L groups, respectively. The equal volume of normal saline was given instead in group PNI. On 1, 2, 4, 8 and 12 weeks after establishment of the model, 5 mice were sacrificed and the nerve tissues were obtained from the site 05 cm up and down the lesion site of the nerve for examination of the shape of the myelin sheath of nerve fibers（with an electronic microscope）.The expression of bFGF in the sciatic nerve tissue was detected by Western blot. Results The shape of medulla sheath was irregular, the thickness of myelin lamellae was thin, the separation of myelin lamellae was aggravate, demyelinate was found, and the proliferation of Schwann cells was not marked in group PNI. The shape of medulla sheath was regular, the thickness of myelin lamellae was dense, and the proliferation of Schwann cells was marked in group H. The shape of medulla sheath was irregular, the separation of mye-lin lamellae was observed, demyelinate was found, and the proliferation of Schwann cells was not marked in group L. Compared with group PNI, the expression of bFGF was significantly up-regulated in H, M and L groups（P〈0.05）. Compared with group L, the expression of bFGF was significantly up-regulated in H and M groups（P〈0.05）. Compared with group M, the expression of bFGF

关 键 词：舒芬太尼 成纤维细胞生长因子2 周围神经 伸经再生 

分 类 号：R614[医药卫生—麻醉学]