噬菌蛭弧菌对医院污水分离细菌捕食作用研究  

Predation efficacy of Bdellovibrio bacteriovorus on bacteria isolated from hospital sewage

作　　者：孙瑶[1] 侯渊博 陈栎江[1] 张艺之 曹建明[3] 周铁丽[1] 

机构地区：[1]温州医科大学附属第一医院医学检验中心,浙江温州325035 [2]浙江大学医学院附属邵逸夫医院检验科,浙江杭州310020 [3]温州医科大学检验医学院生命科学学院,浙江温州325035

出　　处：《中国卫生检验杂志》2017年第24期3534-3536,共3页Chinese Journal of Health Laboratory Technology

基　　金：温州市科学技术局资助项目(Y20140722);浙江省大学生科技创新活动计划暨新苗人才计划(2015R413063)

摘　　要：目的研究医院污水细菌分布情况及噬菌蛭弧菌对分离细菌的捕食作用。方法采用定量接种法对温州地区两家综合性医院污水出水口样本进行菌量计数,并采用VITEK 2 Compact细菌鉴定仪对分离细菌进行鉴定;将噬菌蛭弧菌与分离菌进行共培养,根据每隔12 h培养后菌落数的变化,判断噬菌蛭弧菌对分离菌的作用。结果两家医院污水中菌量分别为2.8×10~5cfu/ml和1.5×10~5cfu/ml,主要分离菌为肺炎克雷伯菌、大肠埃希菌、蜡样芽胞杆菌、弧菌属细菌、普通变形杆菌等;噬菌蛭弧菌对大肠埃希菌、肺炎克雷伯菌和弧菌属细菌具有一定的捕食作用,经过72 h的共培养,大肠埃希菌、肺炎克雷伯菌和弧菌属的菌量均发生约3 log10降低;对变形杆菌及蜡样芽胞杆菌未出现捕食作用。结论噬菌蛭弧菌可捕食部分革兰阴性菌,提示其可作为一种生物消毒剂应用于医院污水处理。Objective To investigate the distribution of bacteria in the hospital waste water and the predation efficacy of Bdellovibrio bacteriovorus on the isolated strains. Methods Bacterial colony counting was conducted on the outlet of waste water from two general hospitals in Wenzhou. The isolated strains were identified by VITEK2 Compact system. The isolated strains were co-cultured with B. bacteriovorus and colony counting was performed every 12 h during the cultivation to predict the predation of B. bacteriovorus on strains. Results The original amount of bacteria in the two hospitals were 2. 8 × 10^-5 cfu/ml and 1. 5 ×10^-5 cfu/ml respectively. The main isolated strains were Klebsiella pneumoniae,Escherichia coli,Bacillus cereus,Vibrio and Proteus vulgaris,etc. We found that the B. bacteriovorus could effectively prey on K. pneumoniae,E. coli,and Vibrio,with a reduction of approximately 3 log10 in viability after 72 h co-culture. The B. bacteriovorus did not show distinct predation efficacy on P. vulgaris and B. cereus. Conclusion The B. bacteriovorus could prey on some gram negative bacteria,suggesting the application of B. bacteriovorus as a biological disinfectant into waste water treatment.

关 键 词：噬菌蛭弧菌 捕食 医院污水 

分 类 号：R446.5[医药卫生—诊断学]