间充质干细胞促进食管鳞癌细胞上皮间质转化  

Human bone marrow mesenchymal stem cells promote epithelial mesenchymal transition in esophageal squamous cell carcinoma cells

作　　者：杨伟林[1] 王涛[2] 吴佳斌[1] 肖婕斐 王儒琛 陈振光[1] 

机构地区：[1]中山大学附属第一医院,广州510000 [2]中山大学干细胞与组织工程研究中心,广州510000

出　　处：《广西医科大学学报》2017年第12期1691-1695,共5页Journal of Guangxi Medical University

基　　金：中国博士后科学基金资助项目(No.2014M552218)

摘　　要：目的:观察人骨髓间充质干细胞(MSCs)对食管鳞癌细胞Eca-109和TE-1上皮间质转化的影响。方法:收集MSCs培养上清,分别用MSCs上清及无血清无双抗培养液作为对照诱导培养食管鳞癌细胞Eca-109和TE-1,观察肿瘤细胞形态学改变;利用qPCR、western blotting法检测Eca-109和TE-1细胞在诱导培养过程中上皮间质转化相关指标E-钙黏素(E-cadherin)、N-钙黏素(N-cadherin)和波形蛋白(Vimentin)的mRNA及蛋白水平的表达变化;利用Transwell实验检测MSCs培养上清对Eca-109和TE-1细胞迁移能力的影响。结果:MSCs上清能诱导Eca-109、TE-1肿瘤细胞形态从上皮细胞形态向间质细胞形态转化。与对照组相比,实验组上皮间质转化相关指标E-cadherin mRNA表达降低,N-cadherin和Vimentin mRNA表达升高;实验组TE-1细胞E-cadherin蛋白表达降低,Vimentin表达升高。Transwell实验显示实验组肿瘤细胞迁移能力明显增强。结论:MSCs能诱导食管鳞癌细胞Eca-109和TE-1发生上皮间质转化,增强食管癌细胞的迁移能力。Objective： To explore the effect of human bone marrow mesenchymal stem cells（MSCs）on epithelial mesenchymal transition in esophageal squamous cell carcinoma（ESCC）Eca-109 and TE-1 cells. Methods：The Eca-109 and TE-1 cells were cultured with MSCs supernatant（MSCs conditioned medium,MSCs-CM）.The cellular morphology was observed under a microscope. The mRNA and protein expressions of E-cadherin,N-cadherin and Vimentin were determined by qPCR and western blotting,respectively. Transwell assay was used to detect the migration ability of cells. Results： Compared with the control group,the cellular morphology of experimental group showed mesenchymal-like changes. In response to MSCs-CM,there was decreased E-cadherin but increased N-cadherin and Vimentin at mRNA and protein levels,compared with the control group（P〈0. 05）. The migration ability of the experimental group was also elevated. Conclusion： MSCs could promote epithelial mesenchymal transition in ESCC Eca-109 and TE-1 cells,and enhance the migration ability of Eca-109 and TE-1 cells.

关 键 词：食管鳞癌 间充质干细胞 上皮间质转化 肿瘤转移 

分 类 号：R318.5[医药卫生—生物医学工程]