三氯生的体外遗传毒性评价  

Genotoxicity evaluation of triclosan in vitro

作　　者：曹易懿[1] 奚晶[1] 唐伟锋 尤馨悦 刘维映 栾洋[1] 

机构地区：[1]上海交通大学医学院虹桥国际医学研究院/公共卫生学院,上海200025 [2]上海大学环境与化学工程学院环境污染与健康研究所,上海200444

出　　处：《癌变．畸变．突变》2018年第1期71-75,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：上海市卫生和计划生育委员会青年项目(20144Y0100)

摘　　要：目的:应用体外碱性彗星试验、体外胞质分裂阻滞微核细胞组学试验和细菌回复突变试验(Ames试验)评价三氯生(TCS)的体外遗传毒性。方法:采用TK6人淋巴母细胞进行体外彗星试验和体外微核试验,共设定5个剂量(3.5、8.8、17.5、26.3和35--μmol/L)组。同时,应用鼠伤寒沙门氏菌TA98、TA100和DNA修复酶缺陷型YG7108(Ogt^-/Ada)菌株对TCS进行Ames试验,共设定8个剂量(0.000 5、0.001 67、0.005、0.016 7、0.05、0.167、0.5和1.67μg/皿)组。结果:与对照组比较,彗星试验结果显示,TCS在各个剂量下均能引起TK6细胞的尾长、尾部DNA强度和尾矩的增加,差异均具有统计学意义(P均<0.01),且尾部DNA强度呈现剂量效应(r=0.943,P=0.017);微核试验表明,TCS未引起TK6细胞微核率升高(P>0.05);Ames试验结果表明TCS未引起TA98、TA100和YG7108菌株的回复突变菌落数增加(P>0.05)。结论:TCS主要引起细胞DNA损伤,但未对TK6细胞造成染色体损伤,对Ames试验菌株不具有致突变作用。OBJECTIVE： Genotoxicity of triclosan was evaluated in TK6 and bacterial cells by alkaline comet assay,cytokinesis-block micronucleus （CBMN） test and bacterial reverse mutation test （Ames test）. METHODS：Comet assay and CBMN test were performed by TK6 cell,and 5 doses of TCS were set as 3.5,8.8,17.5,26.3 and 35 μmol/L. - -Three Salmonella typhimurium tester strains TA98,TA100 and YG7108 （DNA repair deficient strain,Ogt /Ada ） were employed to perform Ames test,and 8 doses of TCS were set at 0.000 5,0.001 67,0.005,0.016 7,0.05,0.167,0.5 and 1.67 μg/plate. RESULTS：The results of comet assay showed that TCS caused DNA damage with a significant increase in comets tail length,tail DNA intensity and tail moment （P〈0.01）,and have a statistical correlation with tail DNA intensity （r=0.943,P=0.017）. The CBMN test indicated that TCS did not induce increased frequency of micronucleus （P〉0.05） and no consistent increase in numbers of revertant colonies of three strains was seen in Ames test （P〉0.05）. CONCLUSION：TCS mainly induced severe DNA injury to TK6 cell in a dose-dependent manner. TCS did not cause chromosome damage and also was not mutagenic to three Salmonella typhimurium strains.

关 键 词：三氯生 彗星试验 微核试验 AMES试验 遗传毒性 

分 类 号：R994.6[医药卫生—毒理学]