绝经后女性血浆骨代谢相关差异蛋白表达的研究  

Research of Protein Different Expression Related to Bone Metabolism of Postmenopausal Women Plasma

作　　者：郑雪峰[1] 吴铅淡 林燕萍[1] 

机构地区：[1]福建中医药大学针灸学院,福州350122

出　　处：《江西中医药大学学报》2018年第2期28-30,50,共4页Journal of Jiangxi University of Chinese Medicine

基　　金：国家自然科学基金项目(81674054);福建省教育厅科技计划项目(JK2015021)

摘　　要：目的:观察绝经后骨质疏松症患者相关骨代谢差异蛋白表达情况,筛选出评价绝经后骨质疏松症严重程度的骨代谢指标。方法:采用悬液芯片技术分析不同骨量绝经后妇女促肾上腺皮质激素(ACTH)、糖蛋白Dickkop(DKK-1)、成纤维细胞生长因子23(FGF-23)、白介素1β(IL-1β)、IL-6、胰岛素(Insulin)、瘦素(Leptin)、骨保护素(OPG)、骨钙素(BGP)、骨桥蛋白(Osteopontin,OPN)、甲状旁腺激素(PTH)、硬骨素(SOST)、肿瘤坏死因子a(TNF-a)蛋白的差别。结果:PMOP患者骨代谢标志物DKK-1、IL-6、BGP水平与骨密度呈负相关,OPG、Leptin、FGF-23、Insulin水平与骨密度呈正相关,且相关性有统计学意义(P<0.05)。结论:人血中BGP、DKK-1、OPG、OPN、IL-6、Leptin、FGF-23、Insulin可作为评价绝经后女性骨质疏松严重程度的检测指标。Objective To observe the expression of bone metabolism protein in postmenopausal osteoporosis patients. Screening of bone metabolic markers that can assess the severity of postmenopausal osteoporosis. Methods： Analysis of ACTH,DKK-1,FGF-23,IL-1β,IL-6,Insulin,Letin,OPG,BGP,OPN,PTH,SOST,TNF-α in postmenopausal women with different bone mass groups by suspension array. Results： DKK-1,IL-6,BGP levels were negatively correlated with bone mineral density in PMOP patients,OPG,Leptin,FGF-23,Insulin levels were positively correlated with bone mineral density in PMOP patients,and the correlation was statistically significant（P 0. 05）. Conclusion： In the plasma of patients with BGP,DKK-1,OPG,OPN,IL-6,Leptin,FGF-23 and Insulin can be used as an index to evaluate the severity of osteoporosis in postmenopausal women.

关 键 词：绝经后骨质疏松症 悬液芯片 骨代谢 

分 类 号：R681.1[医药卫生—骨科学]