HPLC法测定柴芍安神解郁颗粒中芍药苷的含量  

Determination of Paeoniflorin in Chaishao Anshen Jieyu Granules by HPLC

作　　者：郭鸣[1,2] 李智勇 李洁环 莫平莉[1,2] 王洛临 陈雪婷 

机构地区：[1]广州中医药大学,广州510405 [2]广东省第二中医院(广东省中医药工程技术研究院),广州510095 [3]广东省中医药研究开发重点实验室,广州510095

出　　处：《江西中医药大学学报》2018年第2期63-64,81,共3页Journal of Jiangxi University of Chinese Medicine

基　　金：广东省科技计划项目(2015A020211020)

摘　　要：目的:建立柴芍安神解郁颗粒中芍药苷的含量测定方法。方法:采用色谱柱Eclipse XDB-C18(250 mm×4.6 mm,5μm),流动相∶乙腈-0.1%磷酸水溶液(14∶86),流速:1.0 m L/min,检测波长:230 nm,柱温:25℃,进样量:10μL。结果:芍药苷在0.12096~1.20960μg范围内线性关系良好,回归方程为Y=1.4294X-36.7473(r=0.999 9),线性关系良好,平均加样回收率为100.39%,RSD为1.58%。结论:所建立的方法简单可行,重复性好,可用于柴芍安神解郁颗粒中芍药苷的含量测定。Objective： To establish a method for the content determination of paeoniflorin in Chaishao Anshen Jieyu Granules. Methods：The samples were analyzed on a Eclipse XDB-C18（250 mm × 4. 6 mm,5 μm） column and the mobile phase consisted of acetonitrile--0. 1% phosphonic acid solution（14∶ 86） at a flow rate of 1. 0 m L/min. The detection wavelength was set at 230 nm,the sample size was 10 μL and the column temperature was 25℃. Results： The calibration curve was linear within the range of 0. 12096 ~ 1. 20960μg for paeoniflorin,and the regression equation was Y = 1. 4294 X-36. 7473（r = 0. 999 9）. The average recovery was 100. 67%,RSD was1. 58 %. Conclusion： This method is simple and reproducible,which can be used for determination of paeoniflorin in Chaishao Anshen Jieyu Granules.

关 键 词：柴芍安神解郁颗粒 芍药苷 含量测定 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]