生物反应器流场特性研究及其在生物过程优化与放大中的应用研究  

Study of fluid dynamics in bioreactors and its application in bioprocess optimization and scale-up

作　　者：夏建业[1,2] 谢明辉 储炬[1,2] 庄英萍 张嗣良[1,2] 

机构地区：[1]生物反应器工程国家重点实验室,华东理工大学生物工程学院,上海200237 [2]国家生化工程技术研究中心(上海),上海200237

出　　处：《生物产业技术》2018年第1期41-48,共8页Biotechnology & Business

摘　　要：生物反应器是实现生物过程的核心设备,其内进行的生物反应过程受不同反应器内流场结构的影响会产生差异显著的结果,这也是导致生物过程放大困难的关键问题之一。为研究这一问题,必须对不同规模反应器内流场结构有充分的认识,必须对反应器内的混合、传质、剪切等影响生物过程的几个关键方面进行深入研究。首先简单介绍了反应器流场研究的实验及数值模拟方法,在此基础上详细论述了流场混合与传质对生物反应过程的影响,流场剪切对丝状菌形态及其生物过程的影响,最后从反应器流场与细胞生理代谢特性整合的角度,介绍了反应器流场与细胞生理之间的相互作用关系,并以三个实例简单介绍了基于Euler-Lagrange模拟框架的整合流场和细胞动力学模型的模拟方法及模拟结果。旨在通过介绍反应器流场研究及其在生物过程优化放大中的应用,提出基于反应器流场特性与细胞动力学响应的生物过程放大研究方法,为更高效、理性地生物过程放大提供一些理论参考。Bioreactor is the key instrument for implementing a bioprocess, and observed discrepant results of bioreaction process carried out in the bioreactor are strongly infl uenced by the fl ow fi eld structure in different bioreactors, which is one of the key problems for bioprocess scaling up. For investigating this issue, the authors should have adequate knowledge about the fl ow fi eld structure in bioreactors with different scale, and should do detailed study on key issues like mixing, mass transfer and shear force, which are thought influencing the bioprocess much. This paper firstly gives a primary introduction of both experimental and numerical method for investigating bioreactor fl ow fi eld, based on this, the infl uence of mixing and mass transfer to the bioprocess, and the effect of shear force on the morphology of fi lamentous strain are discussed in detail. At last, the interrelationship between fl ow fi eld and cell physiology is introduced from the aspect of integration of fl uid dynamics and cell physiological properties. Three examples of applying integration of fl ow fi eld and cell kinetics by using Euler-Lagrange simulation frame are given at the end. This paper aims at proposing the scale-up method based on both bioreactor fl ow fi eld and cell kinetics response by the topic of fl uid dynamics study in bioreactor and its application in bioprocess scaling up. The authors hope this can provide some basic theory reference for more effi cient and rational bioprocess scale-up.

关 键 词：搅拌生物反应器 发酵过程放大 计算流体力学 细胞动力学 scale-down反应器 

分 类 号：Q81[生物学—生物工程]