内生木霉P3.9菌株的多功能性及其枇杷根腐病的盆栽防效  

Multifunction of Endophytic Trichoderma P3.9 Strain and Control Effect on Loquat Root Rot

作　　者：鲁海菊[1] 沈云玫[1] 陶宏征[1] 江涛 王传铭[1] 

机构地区：[1]红河学院生命科学与技术学院,云南蒙自661199

出　　处：《西北农业学报》2017年第11期1681-1688,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(31660147);云南省应用基础研究计划(2016FB066);红河学院应用型科学研究重点项目(XJY15Z06)~~

摘　　要：为明确内生木霉P3.9菌株多功能性及其生防潜力,采用菌丝生长速率法、透明圈法、农艺性状观测法、组织分离法、土壤梯度稀释法和盆栽法,分别测定其对不同植物病原菌的抑制率、不同化学农药的耐药性、纤维素及磷的降解能力、促生作用、在枇杷植株及其根际土壤中的定殖能力和枇杷根腐病的防治效果。结果表明,内生木霉P3.9菌株对7种植物病原菌有不同程度的抑菌作用,其中,对枇杷根腐病菌的抑制作用最强,抑菌率达93%;对5种化学农药有不同程度的耐药性,其中,对硫磺三唑酮的耐药性最高;对纤维素及磷有极强的降解能力;对枇杷植株有促生作用,能定殖于枇杷植株及其根际土壤中,盆栽防治枇杷根腐病效果可达80%。In order to determine the multifunction characteristics and biocontrol potential of endophytic Trichoderma P3.9 strain,Abilities of inhibiting different plant pathogens,resistance to different fungicides,degradation of cellulose and calcium phosphate,growth-promoting,colonization and control of loquat root rot were tested by methods of mycelial growth rates,transparent zone,observation of agronomic trait,tissue isolation of plant,gradient dilution of soil and pot-culture.The results showed that the P3.9 strain had inhibiting effect on seven kinds of plant pathogens.Moreover,the strongest inhibiting effect was detected on loquat root rot,with the inhibition rate of 93%.The P3.9 strain had also a degree of resistance to seven kinds of fungicides,and the best resistance effect was detected on sulfur-triadimenol.It had strong ability of degrading cellulose and calcium phosphate,promoting growth of loquat plant.The P3.9 strain was colonize in loquat plant and rhizospere soil.Control effect of P3.9 strain on loquat root rot was 80%.

关 键 词：内生木霉 拮抗作用 耐药性 降解纤维素能力 解磷能力 促生作用 定殖能力 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]