Expression of E2A in Mid-secretory Endometrium of Women Suffering from Recurrent Miscarriage  

作　　者：尹智囊 丁锦丽 张怡 李赛姣 张艳 杨菁 

机构地区：[1]Department of Histology and Embryology,Medical College of Wuhan University,Wuhan 430071,China [2]Reproductive Medical Center,Renmin Hospital of Wuhan University,Wuhan 430060,China [3]Hubei Clinic Research Center for Assisted Reproductive Technology and Embryonic Development,Wuhan 430060,China [4]Department of Clinical Laboratory,Renmin Hospital of Wuhan University,Wuhan 430060,China

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2017年第6期910-914,共5页华中科技大学学报（医学英德文版）

基　　金：supported by the grants from National Natural Science Foundation of China(No.81401276,No.81771618 and No.81771662);the Fund Project of Health and Family Planning Commission of Hubei Province(No.WJ2015MA006 and No.WJ2015Q017)

摘　　要：E2A is involved in promoting forkhead box P3（FOXP3） and retinoid-related orphan receptor gamma t（RORγt） gene transcription, which are pivotal transcription factors of T regulatory cells and Th17 cells, respectively. Little is known about the involvement of E2 A in pregnancy process. This study aimed to investigate the expression of E2 A, cytotoxic T-lymphocyte-associated protein 4（CTLA-4）, and Foxp3 in luteal phase endometrium of women suffering recurrent miscarriage（RM）（n=21） and control group（n=11） by immunohistochemistry, with the Vectra？ automated quantitative pathology imaging system for analysis. The percentage of E2 A＋ cells and CTLA-4＋ cells was significantly higher in the endometrium of women with RM than in the controls. There was positive correlation between E2 A and CTLA-4（r=0.523, P=0.002）, E2 A and FOXP3（r=0.380, P=0.032）, and FOXP3 and CTLA-4（r=0.625, P=0.000） in the mid-secretory phase of endometrium for all subjects. It was concluded that the abnormal expression of endometrial E2 A existed in mid-secretory endometrium of women with RM, and there was a positive correlation between E2 A and FOXP3, and E2 A and CTLA-4, suggesting the possible regulation role of E2 A involved in regulating endometrium receptivity.E2A is involved in promoting forkhead box P3（FOXP3） and retinoid-related orphan receptor gamma t（RORγt） gene transcription, which are pivotal transcription factors of T regulatory cells and Th17 cells, respectively. Little is known about the involvement of E2 A in pregnancy process. This study aimed to investigate the expression of E2 A, cytotoxic T-lymphocyte-associated protein 4（CTLA-4）, and Foxp3 in luteal phase endometrium of women suffering recurrent miscarriage（RM）（n=21） and control group（n=11） by immunohistochemistry, with the Vectra？ automated quantitative pathology imaging system for analysis. The percentage of E2 A＋ cells and CTLA-4＋ cells was significantly higher in the endometrium of women with RM than in the controls. There was positive correlation between E2 A and CTLA-4（r=0.523, P=0.002）, E2 A and FOXP3（r=0.380, P=0.032）, and FOXP3 and CTLA-4（r=0.625, P=0.000） in the mid-secretory phase of endometrium for all subjects. It was concluded that the abnormal expression of endometrial E2 A existed in mid-secretory endometrium of women with RM, and there was a positive correlation between E2 A and FOXP3, and E2 A and CTLA-4, suggesting the possible regulation role of E2 A involved in regulating endometrium receptivity.

关 键 词：E2A cytotoxic T-lymphocyte-associated protein 4 forkhead box P3 retinoid-related orphan receptor gamma t recurrent miscarriage 

分 类 号：R714.21[医药卫生—妇产科学]