胞嘧啶保护银纳米簇的合成及其成像研究  

Synthesis of Cytosine Templated Silver Nano Clusters and Cell Imaging

作　　者：爱军[1,2] 嘎鲁 

机构地区：[1]内蒙古师范大学化学与环境科学学院,内蒙古呼和浩特010022 [2]内蒙古自治区功能材料物理与化学重点实验室,内蒙古呼和浩特010022 [3]内蒙古医科大学药学院,内蒙古呼和浩特010110

出　　处：《内蒙古师范大学学报（自然科学汉文版）》2017年第6期869-873,共5页Journal of Inner Mongolia Normal University(Natural Science Edition)

基　　金：内蒙古自然科学基金资助项目(2013MS0217);电分析国家重点实验室开放课题(SKLEAC201503);内蒙古医科大学科技百万项目(YKD2016KJBW019)

摘　　要：利用胞嘧啶(C碱基核苷酸)、硼氰化钠(NaBH_4)、硝酸银(AgNO_3)为原料合成了含不同C碱基保护的银纳米簇,并采用荧光光谱、圆二色谱(CD)、透射电镜和激光共聚焦显微镜对C碱基核苷酸保护的银纳米簇进行了检测和表征.荧光光谱分析发现,C碱基核苷酸保护的银纳米簇有良好的荧光,透射电镜结果表明纳米簇颗粒小于2nm,共聚焦成像结果说明胞嘧啶保护银纳米簇能进入癌细胞,同时能细胞成像.Herein,C base templated fluorescent silver nanoclusters are synthesized and successfully applied for cancer cell imaging and MTT assay.C base protected silver nanoclusters were obtained by C base,boron sodium cyanide（NaBH_4）and silver nitrate（AgNO_3）.We used fluorescent assay,CD and TEM to characterize the silver nanoclusters.It is found that after formation of the silver nanocluster,it has good fluorescence optical characteristics.The TEM image reveals that the spherical nanoparticles can be clearly distinguished due to the high elctron density of the molecule,and the size of silver nanocluster is smaller than 2 nm.Confocal imaging results indicated that C base protected silver nanoclusters could enter cancer cell and cell fluorescence imaging.

关 键 词：胞嘧啶 银纳米簇 HELA细胞 生物成像 

分 类 号：O641.4[理学—物理化学]