马比木不同部位喜树碱含量测试分析  

Camptothecin Contents and Transfer Process in Different Parts of Nothapodytes Pittosporoides

作　　者：高渐飞 张凡 杨艳[2] 张明[2] 

机构地区：[1]贵州省山地资源研究所,贵州贵阳550001 [2]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550002

出　　处：《绿色科技》2018年第1期1-3,5,共4页Journal of Green Science and Technology

基　　金：贵州省科技支撑计划项目(编号:黔科合SY字[2014]3060号)

摘　　要：分析了马比木不同部位喜树碱含量及其运移过程,采用高效液相色谱法对其进行了测定。结果表明:色谱柱Syncyronis C18(4.6mm×250mm,5μm);流动相:甲醇-水(60∶40),等度洗脱;监测波长:254nm;流速:0.8mL/min;柱温:30℃。测定结果表明:马比木喜树碱含量为叶=嫩枝皮<嫩枝木质部<主枝皮<主枝木质部<主干皮<主干木质部<根木质部<根树皮。不同部分喜树碱含量差异较大,根部到地上部分至植株顶端喜树碱含量逐渐降低,不同部位树皮喜树碱含量差异比木质部大。This paper analyzedcamptothecin content and transfer process in different parts ot nothapoclytesplt- tosporoides. High performance liquid chromatography was used to measure Chromatographic column= SyncyronisC18 （4.6mm250 mm, 5？ m）; mobile phase= methanol-water （60：40）, isocratic elution; monitoring wavelength= 254nm; flow rate： 0. 8ml/min; column temperature= 30. The result showed that the camptothecin contents in nothapodytespittosporoidesareleaf： twig bark〈xylem of twig bark〈bough bark〈xylem of bough〈trunk bark〈xy- lem of trunk〈xylem of root〈2root bark. Camptothecin content varies more significantly from part to part, decreasing gradually from the aboveground part of the root to the top of the plant. Compared to xylem, the camptothecin content differences between the bark of different parts are greater .

关 键 词：马比木 喜树碱 含量 差异 

分 类 号：S759.35[农业科学—森林经理学]