大豆转录因子GmbZIP60对非生物胁迫的表达模式分析  

Expression Patterns of Soybean Transcription Factor GmbZIP60 in Response to Abiotic Stresses

作　　者：邢锦城[1] 孙晨曦 洪立洲[1] 许玲[3] 徐照龙[3] 张大勇[3] 

机构地区：[1]江苏沿海地区农业科学研究所,江苏盐城224002 [2]山东省环境规划研究院,山东济南250101 [3]江苏省农业科学院农业资源与环境研究所,江苏南京210094

出　　处：《大豆科学》2018年第1期45-49,共5页Soybean Science

基　　金：江苏省农业科技自主创新项目[CX(15)1005];江苏省自然科学基金面上项目(BK20151301)

摘　　要：bZIP基因在植物响应非生物胁迫过程中发挥重要的调控作用。前期试验已经从大豆中克隆到一个b ZIP基因Gmb ZIP60(Genebank Accession No:DQ787038),并成功转化到拟南芥中。本研究在此基础上,利用q RT-PCR和GUS组织化学染色法研究了高温(37℃)、低温(4℃)、干旱(PEG6000)、NaCl(200 mmol·L^(-1))和ABA(100 mg·L^(-1))处理对Gmb ZIP60表达的影响。结果表明:高温、低温、干旱和Na Cl胁迫处理下,Gmb ZIP60表达量显著降低,分别变为对照组的0.07,0.25,0.36和0.13倍,而ABA处理对Gmb ZIP60表达量影响不显著,GUS染色结果与荧光定量PCR结果一致。研究认为,Gmb ZIP60可能以负调控的方式参与大豆抗高盐、干旱、高温、低温等非生物胁迫的应答反应。本研究为进一步鉴定和克隆大豆逆境应答关键基因、揭示大豆抗逆分子机制和大豆抗逆性基因工程改良提供了有益借鉴。bZIP genes play significant roles in the response to abiotic stresses. We have cloned a bZIP gene named as GmbZ- IP60 （ Genebank, Accession, No： DQ787038） from soybean, and transformed it into Arabidopsis thaliana. Then on the base of these, this paper studied the GmbZIP60 gene expression profiling in Arabidopsis seedlings under the treatments of PEG, high- temperature（37℃）, low-temperature（4℃）, high-salt（PEG6000） and ABA（ 100 mg.L-1） by qRT-PCR and GUS histo-chemical staining. The fluorescence quantitative RT-PCR results showed that the expression of GmbZIP60 genes were down-regulated by PEG, high-temperature, low-temperature and salt treatments respectively. The results of GUS staining were agree with fluorescence quantitative RT-PCR. This indicated that GmbZIP60 might be involved in the responsing to abiotic stresses such as high salinity, drought, high temperature and low temperature. This study laid the foundation for revealing the molecular mechanism of soybean resistance and genetic engineering improvement of soybean resistance.

关 键 词：大豆 bZIP转录因子 非生物胁迫 

分 类 号：S565.1[农业科学—作物学]