大豆异黄酮对小鼠肝癌移植瘤血管生成的抑制作用  

Anti-angiogenic Effect of Soy Isoflavones in H_(22)-transplanted Mice

作　　者：郑峰[1] 崔香丹[1] 朱洁波 尹学哲[1] 

机构地区：[1]延边大学附属医院,吉林延吉133000

出　　处：《大豆科学》2018年第1期101-104,共4页Soybean Science

基　　金：国家自然科学基金(81360651;81760659)

摘　　要：为了探讨大豆异黄酮对肝癌移植瘤血管生成的抑制作用,建立小鼠H22肝癌皮下移植瘤模型。用大豆异黄酮干预10 d,利用免疫组化染色法检测肿瘤组织内微血管密度,利用免疫印迹法检测肿瘤组织内血管内皮细胞生长因子(VEGF)、血管内皮细胞生长因子受体2(VEGFR2)和缺氧诱导因子-1α(HIF-1α)的表达,酶联免疫吸附测定(ELISA)法检测转化生长因子-β1(TGF-β1)和基质金属蛋白酶2(MMP2)的水平。结果显示,大豆异黄酮组移植瘤细胞生长受到抑制,细胞坏死较严重。与模型组比较,大豆异黄酮组肿瘤组织内微血管密度降低;VEGF、VEGFR2和HIF1α的蛋白表达降低;TGF-β1及MMP2蛋白表达下降。提示,大豆异黄酮对小鼠H22移植瘤组织的血管生成具有抑制作用,机制可能与下调移植瘤组织VEGF和TGF-β1蛋白表达有关。The anti-angiogenic effect of soy isoflavones in hepatoma-transplanted mice was investigated. Mice bearing H2 heparoma cells were established and treated with soy isoflavones for 10 days. The microvessel density was detected using immunohistochemical staining, the expression of vascular endothelial growth factor （VEGF）, vascular endothelial growth factor receptor2 （VEGFR2） and hypoxia-inducible factor-1α （HIF-1α） was detected by the western blot method, and the transforming growth factor-β1 （TGF-β1） and matrix metallopmteinase2 （MMP2） were analyzed with the enzyme-linked immunosorbent assay （ELISA） method. Soy isoflavones decreased the microvessel density of tumor tissue, and down-regulated the protein expression of VEGF, VEGFR-2, HIF-1α, TGF-β1 and MMP2 in transplanted tumor tissue. Taken together, soy isoflavones could exhibit the anti-angiogenic effect in H22-transplanted mice, and its mechanism is probably related to down-regulation of VEGF and TGF-β1 proteins.

关 键 词：大豆异黄酮 H22 抗血管生成 

分 类 号：S565.1[农业科学—作物学] R285.5[医药卫生—中药学]