母体密度应激与球虫感染对子代根田鼠免疫力的影响  

Effects of maternal density stress and coccidiosis infection on immunity in root vole offsprings

作　　者：张鑫 杨彦宾[1,2] 堵守杨 曹伊凡[1,3,4] 周华坤[1,5] 边疆晖 

机构地区：[1]中国科学院西北高原生物研究所,西宁810001 [2]中国科学院大学,北京100049 [3]中国科学院高原生物适应与进化重点实验室,西宁810001 [4]青海省动物生态基因组学重点实验室,西宁810001 [5]青海省寒区恢复生态学重点实验室,西宁810001

出　　处：《兽类学报》2018年第1期56-65,共10页Acta Theriologica Sinica

基　　金：国家自然科学基金(31570421);国家重点研发计划项目(2016YFC0501902)

摘　　要：免疫功能是影响动物个体适合度的重要因素之一,也是防御病原体入侵的重要途径,对动物的生存至关重要。种群密度与寄生物感染都能影响动物的免疫功能。本实验通过2×2析因实验设计,测定了母体密度应激+球虫感染、母体密度应激+未球虫感染、未母体密度应激+球虫感染、未母体密度应激+未球虫感染4个处理对根田鼠子代粪便皮质酮、球虫感染率、白细胞总数、各型白细胞的百分比、淋巴细胞亚型计数(CD4,CD8计数)等指标。结果表明,高密度母体应激处理可显著降低CD4数量、CD4/CD8比例、淋巴细胞数、嗜酸性粒细胞数、白细胞数,母体密度应激与球虫的耦合可使上述免疫指标进一步降低,说明母体密度应激和球虫感染对于根田鼠的免疫具有负的叠加效应。The immune function is one of the most important factors to influence animals fitness. As it is a crucial way to defend against bacterial pathogens such as viruses,the immune system plays a key role in animal’s survival. Both population density and parasitization are known to deteriorate individuals’ immune function. This experiment using 2 × 2 factorial experiment design to measure the indexes of four treatments（ maternal density stress ＋ coccidian infection,not maternal density stress ＋ not coccidian infection,maternal density stress ＋ not coccidian infection,not maternal density stress ＋ coccidian infection）,including the fecal corticosterone metabolite（ FCM）,coccidian infection rate,blood parameters,lymphocyte subsets counts（ CD4,CD8 counts）. The results show that the density-induced maternal stress can significantly reduce the number of CD4,rate of CD4/CD8,Lymphocytes,Eosinophils,white cell. The effects were further deteriorated when maternally stressed offspring infected with coccidian. Our results showed that the interaction of density-induced maternal stress and coccidian infection has negatively synergistic effect on the immune of root vole.

关 键 词：根田鼠 母体应激 球虫 淋巴细胞亚型 

分 类 号：Q494[生物学—生理学]