HS-SPME-GC/MS法分析中缅树鼩尿液的化学成分  

Analysis of the chemical components in the urine of the tree shrew (Tupaia belangeri) by HS-SPME-GC/MS

作　　者：叶芳艳 左木林 朱万龙[1] 高文荣[1] 张浩[1] 侯东敏[1] 王政昆[1] 

机构地区：[1]云南省高校西南山地生态系统动植物生态适应进化及保护重点实验室云南师范大学生命科学学院,昆明650500

出　　处：《兽类学报》2018年第1期85-94,共10页Acta Theriologica Sinica

基　　金：国家国际科技合作专项(2014DFR31040);国家自然科学基金项目(31660121);云南省科技计划项目(2015GA008);云南省应用基础研究计划重点项目(2016FA045)

摘　　要：尿液是动物新陈代谢的产物,在动物的化学通讯中发挥着重要作用,动物依靠它识别个体,选择高质量的配偶,判断亲缘关系,吸引异性等,以增加个体的适合度（Macdonald and Brown,1985）。各种动物尿液的化学成分复杂,含有挥发性和非挥发性两类化学信号物质。尿液的化学组成和其功能在许多动物中已进行报道,如大熊猫（Ailuropoda melanoleuca）（刘玉良等,2012）、Animal urine plays a very important role in animal life. The detection of chemical compounds in Tupaia belangeri urine is the key to identify its composition and assay the function of each chemical compound. In the present study,headspace-solid phase microextraction（ HS-SPME） and gas chromatography-mass spectrometry（ GC-MS） were used to analyze the urine compositions in Tupaia belangeri. Through searching the mass spectrometry library of the National Institute of Standards and Technology（ NIST）,we found that the chemical compounds belong mainly to aldehydes,ketones,acids,esters,aromatic hydrocarbons,hydrocarbons,and phenols,etc. The results showed that HS-SPME-GC/MS is an effective method to detect volatile and semi-volatile compounds in tree shrew urine. In addition,wild individuals with relatively abundant food sources have more components than do those fed on an artificial diet. There are sex differences in the chemical compositions of urine. The results provide a foundation for studying the mechanism of chemical communication in tree shrews.

关 键 词：中缅树鼩 尿液 化学通讯 化学成分 

分 类 号：Q503[生物学—生物化学]