秀丽高原鳅胚胎发育观察  

Observation of Embryonic Development of Triplophysa venusta

作　　者：梁祥 钟文武 冷云 李光华 张宇 左鹏翔 王志飞 张建斌 缪祥军 崔丽莉 姜志武 

机构地区：[1]云南省渔业科学研究院,云南昆明650111 [2]云南华电鲁地拉水电有限公司,云南昆明650228

出　　处：《水产科学》2018年第1期79-84,共6页Fisheries Science

基　　金：国家科技基础条件平台水产种质资源平台项目(2016DKA30470);云南省技术创新人才培养专项(2013HB125);金沙江鲁地拉水电站科研项目[LDL2011/023(P)]

摘　　要：2015年7月,在大理州鹤庆县黑龙潭采集性腺成熟的秀丽高原鳅个体200尾,在水温17.0℃条件下,以促黄体素释放激素类似物5μg/kg+人绒毛膜促性腺激素1000IU/kg的药剂背部肌肉一次注射(雌鱼0.2mL,雄鱼减半),获卵1517粒,效应时间约24h。秀丽高原鳅卵呈黏性,成熟卵粒卵径为1.03mm,发育至眼囊出现时达到1.33mm,在水温(17.5±1.0)℃条件下,观察受精卵的胚胎发育过程,经历卵胚盘形成阶段、卵裂阶段、囊胚阶段、原肠胚阶段、神经胚形成阶段、器官形成阶段和孵化出膜阶段共7个阶段,经约77.75h孵化,开始出膜,出膜后仔鱼全长3.66mm,卵黄经约120h吸收后,开始平游觅食。In July 2015,200 individuals mature Triplophysa venusta sampled in black dragon pool,Heqing County of Dali,Yunnan,was induced to spawn by a muscutar single injection of LRH-A25μg/kg＋HCG 1000 IU/kg with 0.2 mL for females and 0.1 mL for males.In effect time of about 24 h,1517 eggs were finally obtained and the adhensive eggs were 1.03 mm in diameter and reached to 1.33 mm with the appearance of optic sac.The embryonic development of fertilized eggs included blastoderm formation stage,cleavage stage,blastula stage,gastrula stage,neurulation stage,organogenesis stage and hatching stage.The larvae hatched 77.75 hafter incubation,with body length of about 3.66 mm,and started initial feeding after exhaust of egg yolk in 120 hincubation at water temperature of（17.5±1.0）℃.

关 键 词：秀丽高原鳅 人工繁殖 胚胎 发育 仔鱼 

分 类 号：S917.4[农业科学—水产科学]