新疆维吾尔族和汉族儿童龋病与人类白细胞抗原-DQB1等位基因多态性的相关性研究  

Association between the dental caries and the human leucocyte antigen DQB1 allele polymorphisms among the Uygur and Han children in Xinjiang

作　　者：张瑞涵[1] 李小兵[2] 王丽萍[1] 刘奕杉[1] 

机构地区：[1]新疆医科大学第一附属医院儿牙预防保健科,乌鲁木齐830054 [2]口腔疾病研究国家重点实验室国家口腔疾病临床医学研究中心四川大学华西口腔医院儿童口腔科,成都610041

出　　处：《华西口腔医学杂志》2018年第1期4-8,共5页West China Journal of Stomatology

基　　金：国家自然科学基金(81560178)~~

摘　　要：目的探讨人类白细胞抗原(HLA)-DQB1等位基因多态性与新疆维吾尔族和汉族儿童龋病的相关性。方法采用聚合酶链式反应-序列特异性引物(PCR-SSP)DNA分型技术对新疆维吾尔族和汉族高龋儿童及健康无龋儿童(n=40)进行HLA-DQB1基因分型,研究HLA-DQB1基因多态性与新疆维吾尔族和汉族儿童龋病的相关性。结果维吾尔族与汉族儿童在HLA-DQB1座位均检出5个特异性基因位点。汉族高龋组患者HLA-DQB1*02等位基因频率(12.5%)显著低于对照组(32.5%),差异有统计学意义(P<0.05,OR=0.297);维吾尔族高龋组患者HLADQB1*05等位基因频率(37.5%)显著高于对照组(17.5%),差异有统计学意义(P<0.05,OR=2.829)。结论新疆维吾尔族和汉族儿童龋病与HLA-DQB1等位基因多态性具有一定的相关性;HLA-DQB1*02基因可能是新疆汉族儿童龋病的保护因子;而HLA-DQB1*05等位基因是新疆维吾尔族儿童龋病的易感基因。Objective This study aims to investigate the association between human leucocyte antigen （HLA）-DQB1 allele polymorphisms and the presence dental caries among the Uygur and Han children in Xinjiang. Methods HLA-DQB1 allele in the Uygur and Han children with dental caries and healthy control in Xinjiang was tested （n=40） using the polymerase chain reaction-sequence specific primer （PCR-SSP） DNA parting technology. Results A total of five specific loci were detected in the HLA-DQB1 locus among the Uygur and Han children. The frequency of the HLA-DQB1＊02 allele in the Han group with severe caries （12.5%） was significantly lower than in the control group （32.5%） （P〈0.05, OR=0.297）. Moreover, the frequency of the HLA-DQB1＊05 allele in the Uygur group with severe caries （37.5%） was significantly higher than in the control group （17.5%） （P〈0.05, OR=2.829）. Conclusion Caries susceptibility among the Uygur and Han children in Xinjiang is related to the HLA-DQB1 allele. The HLA-DQB1＊02 allele may protect against caries among the Han children, whereas the HLA-DQB1＊05 allele may be responsible for the susceptibility of the Uygur children to caries.

关 键 词：低龄儿童龋 人类白细胞抗原 基因多态性 

分 类 号：Q786[生物学—分子生物学]