水分胁迫下水稻对褐飞虱转录组的影响  

Effects of water-stressed rice on the brown planthopper transcriptome

作　　者：谭椰 徐文彦[1,2] 周文武 鹿东东[2] 商晗武 祝增荣 

机构地区：[1]浙江大学昆虫科学研究所,水稻生物学国家重点实验室,农业部农业昆虫学重点开放实验室,杭州310058 [2]中国计量大学生命科学学院,浙江省生物计量及检验检疫技术重点实验室,杭州310018

出　　处：《植物保护学报》2017年第6期973-981,共9页Journal of Plant Protection

基　　金：国家“973”计划(2010CB126200);浙江省重点科技创新团队项目(2010R50028)

摘　　要：为明确褐飞虱Nilaparvata lugens(St?l)在稻田缺水条件下的发生和灾变规律,以感虫水稻品种TN1为材料,以取食无水分胁迫水稻的褐飞虱为对照组,取食水分胁迫(以15%PEG6000营养液处理48 h模拟)水稻的褐飞虱为处理组,采用Illumina转录组测序技术研究水分胁迫下水稻对褐飞虱转录组的影响。结果显示,测序序列经拼接后得到褐飞虱全部单基因unigene共47 335条,平均长度为1 234 bp;与对照组相比,处理组中表达量差异显著的基因共有1 331条,其中表达量上调的基因有712条,表达量下调的基因有619条;随机选取20条具有显著表达差异的基因,通过实时荧光定量PCR方法检测其表达量以进行验证,其中有13条基因的表达量与测序结果变化一致。对差异表达基因进行GO注释和KEGG代谢通路功能富集分析表明,能量代谢和水分代谢相关通路在水分胁迫处理的褐飞虱中显著富集,表明这几类基因在褐飞虱应答水分胁迫中发挥了重要作用。To explain the rule of outbreak and catastrophe of Nilaparvata lugens （Stal） （brown planthopper, BPH）, the insects used for the sequencing were reared on the rice variety TN1 that were subjected to two levels of water stress using polyethylene glycol （PEG6000） at concentrations of 0 and 15% in 48 h, respectively. Insects reared on plants without water stress was the control. Illumina technology de novo transcriptome sequencing was used to study the effect of water stress on BPH. Transcriptome analysis obtained 47 335 functional unigenes by sequence assemblage, with a mean length of 1 234 bp. Compared with the control, there were 1 331 unigenes showing significantly different expression in water-stressed BPH. Among them, up-regulated and down-regulated transcripts were 712 and 619, respectively. Twenty of the significant differentially expressed genes were randomly selected for verifying the sequencing results by using the real-time qPCR. The results showed that the expression change trends of 13 genes were consistent with the results of transcriptome sequencing. The Gene Ontology （GO） annotation and KEGG pathway analysis of significant difference expressed genes revealed that energy and water metabolism pathways were enriched in the water-stressed BPH, suggesting these genes might play crucial roles in the water-stress response in BPH.

关 键 词：褐飞虱 转录组测序 水分胁迫 基因表达 实时荧光定量PCR 

分 类 号：S435.112.3[农业科学—农业昆虫与害虫防治]