洋甘菊的组织培养技术  

Tissue Culture Technique for Matricaria chamomilia

作　　者：刘晓梦[1] 孟想想 张威威[1] 廖咏玲[1] 常杰 许锋[1] 

机构地区：[1]长江大学园艺园林学院,湖北荆州434025 [2]靶向抗肿瘤药物湖北省协同创新中心,湖北荆门448000 [3]荆楚理工学院化工与药学院,湖北荆门448000

出　　处：《北方园艺》2018年第2期72-76,共5页Northern Horticulture

基　　金：国家自然科学基金资助项目(31400603);大学生创新创业训练计划资助项目(104892014043);荆州市科技发展计划资助项目(2015Z21015)

摘　　要：以洋甘菊(Matricaria chamomilla L.)的嫩茎和幼叶为外植体,采用单因子试验方法,研究不同消毒措施、不同激素浓度配比对洋甘菊愈伤组织诱导的影响,以期为建立洋甘菊再生体系以及通过基因工程手段改良洋甘菊提供参考依据。结果表明:洋甘菊幼嫩的茎段为组织培养的最佳外植体材料,愈伤组织的诱导率高达90%;以幼嫩茎段为外植体,最佳灭菌措施为新鲜的嫩茎用无菌水冲洗30min,75%的酒精浸泡10s,3.5%的次氯酸钠浸泡10min,诱导率高达86.7%,污染率低至13.3%;其愈伤组织诱导的最佳培养基为MS+6-BA 1.5mg·L^(-1)+NAA 1.2mg·L^(-1)。Young stems and young leaves of Matricaria chamomilia were used as explants,by adopting the method of single factor experiment,the influence of different disinfection measures and different hormone concentrations on callus induction of M.chamomilla was studied,in order to establish M.chamomillaregeneration system and genetic engineering methods.The results showed that young stems were the best explants for tissue culture with a high level of callus forming rate of 90%.The best condition of sterilization with young stems was as follow：fresh young stems wash under sterile water for 30 minutes,followed with 75% alcohol for 10 seconds and then 3.5% sodium hypochlorite for 10 minutes,the induction rate was as high as 86.7%,pollution rate was as low as 13.3%;the best medium for callus induction was MS ＋ 6-BA 1.5 mg·L^-1＋ NAA 1.2 mg·L^-1.

关 键 词：洋甘菊 组织培养 诱导率 激素 消毒措施 

分 类 号：S567.239[农业科学—中草药栽培]