直接进样原子荧光法对小鼠肝脏和尿硒含量测定  

Direct sampling introduction and atomic fluorescence spectrometry for determination of selenium content inmice liver and urine

作　　者：陈金丽[1] 冯礼[2] 戚仓 李永芳[1] 王达[1] 李冰[1] 孙贵范[1] 

机构地区：[1]中国医科大学公共卫生学院环境与慢病研究中心,沈阳110122 [2]北京吉天仪器有限公司,北京100016

出　　处：《中华地方病学杂志》2018年第2期149-151,共3页Chinese Journal of Endemiology

基　　金：国家重大科学仪器设备开发专项（2011YQ140149）;国家自然科学基金（81502764）

摘　　要：目的通过对染硒小鼠肝脏和尿硒含量的测定，建立直接进样原子荧光法，为硒生物样品的检测提供精准、便捷的测定方法。方法采用电热石英管释出样品中的硒，原子态硒被石英管捕获，加热石英管释出的硒由氩氢混合气载带进入氩氢火焰原子荧光检测器中进行测定；由硒含量与荧光面积绘制标准曲线，进而计算样品中硒含量。结果直接进样原子荧光法检测生物样本中硒含量的检出限为O．28μg／kg，标准曲线相关系数为0．9993。相对标准偏差为1．82％～4．19％；小鼠肝脏的加标回收率为87．30％～100．20％，尿样的加标回收率为93．10％～96．60％。结论直接进样原子荧光法操作简便易行，准确度和精密度均较好，线性范围宽。待检样品无需经过加酸等复杂的前处理。避免了元素损失，提高了检出效率，可应用于样品中微量硒的快速检测。Objective Through determination of selenium content in liver and urine of selenium-induced mice, direct sampling atomic fluorescence spectrometry was established to provide a more accurate and convenient determination method for detection of selenium-related biological samples. Methods Selenium in the sample was released by the electrically heated quartz tube, the selenium in the atomic state was captured by the quartz tube, and the selenium released by the heating quartz tube was carded by the argon-hydrogen mixed gas into the argon-argon flame atomic fluorescence detector for determination; standard curve was established based on selenium content and fluorescence area, and then the content of selenium in the sample was calculated. Results The detection limit of selenium in samples by direct sampling atomic fluorescence spectrometry was 0.28 μg/kg, the correlation coefficient of standard curve was 0.999 3, and the relative standard deviation range was 1.82% - 4.19%. The adding standard recovery of the liver in mice was 87.30% - 100.20%; meanwhile the adding standard recovery of the urine in mice was 93.10% - 96.60%. Conclusions Direct sampling atomic fluorescence method is simple and easy to operate, accuracy and precision are better, the linear range is wide. The samples need not be processed by complex pretreatment, such as acid, etc., elements loss is avoided and efficiency of detection is improved. The method can be used in a variety of samples for rapid detection of trace selenium.

关 键 词：直接进样 原子荧光光谱法 肝脏 尿 硒 

分 类 号：O657.31[理学—分析化学] R446.1[理学—化学]