Ghrelin及其受体和酰基化酶在羔羊不同组织的表达差异研究  

Study on Differential Expressions of Ghrelin and Its Receptor and Ghrelin O-acyltransferase in Different Tissues in Lambs

作　　者：赵红琼[1] 王琴[1] 徐新明[2] 付强[1] 史慧君[1] 侯宇[1] 杨阳[1] 

机构地区：[1]新疆农业大学动物医学学院,新疆乌鲁木齐830052 [2]新疆畜牧科学院畜牧研究所,新疆乌鲁木齐830000

出　　处：《动物医学进展》2018年第2期49-54,共6页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31560684);新疆农业大学自治区级产学研联合培养研究生示范基地项目(xjaucxyyjs-20141001)

摘　　要：采用real-time PCR检测生长激素释放肽(Ghrelin)及其受体(GHS-R)和酰基化转移酶(GOAT)在羔羊下丘脑、垂体、瘤胃、皱胃底、皱胃窦、十二指肠、空肠、回肠、心、肝、脾、肾、胰腺、睾丸的mRNA表达水平。结果显示,Ghrelin、GHS-R和GOAT mRNA在羔羊各组织中均有表达,其中Ghrelin mRNA主要表达于皱胃底(P<0.01),其次是十二指肠和胰腺(P<0.05);GHS-R mRNA主要表达于垂体(P<0.01),其次是下丘脑(P<0.05);GOAT mRNA在皱胃底和睾丸的表达水平相对较高,均显著高于其他组织(P<0.05)。研究结果表明,Ghrelin系统在反刍动物组织中广泛分布,Ghrelin可能与GHS-R和GOAT共同参与协调羔羊生长调控、摄食等功能的调节作用,为进一步研究反刍动物体内Ghrelin的生物学功能奠定了基础。This study aimed to detect the expressions and distribution pattern of Ghrelin and its receptor（growth hormone secretagogue receptor,GHS-R） and Ghrelin O-acetyltransferase （GOAT） mRNA in hy-pothalamus, pituitary, rumen, abomasum, duodenum, jejunum, ileum, heart, liver, spleen, kidney, pancreas,testis in lambs. The real-time PCR results showed that Ghrelin,GHS-R and GOAT mRNA were expressedin the tissues. Ghrelin mRNA was mainly expressed in the abomasum fundus（P〈0. 01） ,followed by duo-denum and pancreas （P〈0.05）. The expression levels of GOAT mRNA in the fundus of abomasum andtestis were higher than that in other tissues （P〈0.01） ,and GHS-R mRNA was significantly higher in thepituitary （P〈0.05）. The present results indicate that Ghrelin system is widely distributed in ruminant tis-sues,and Ghrelin,GHS-R and GOAT may be involved in the regulation of growth and food intake in rumi-ants.

关 键 词：羔羊 生长激素释放肽 生长激素促分泌素受体 Ghrelin酰基化转移酶 实时荧光定量PCR 

分 类 号：S826[农业科学—畜牧学]