病死猪大肠埃希菌与变形杆菌的分离鉴定和药敏试验  

Isolation,Identification and Drug Sensitivity Test of Escherichia coli and Proteus from Dead Pigs

作　　者：路振香[1] 孙坤[1] 夏礼贤 郭伟娜[1] 刘畅[1] 

机构地区：[1]安徽科技学院动物科学学院,安徽凤阳233100

出　　处：《动物医学进展》2018年第2期134-136,共3页Progress In Veterinary Medicine

基　　金：安徽科技学院兽医学重点学科建设项目(AKZDXK2015A04);安徽省科技攻关计划项目(1301ZC03088)

摘　　要：为弄清安徽五河某养猪场送检病死猪的病因,采集病死猪肝脏组织进行病原分离培养,生化试验及药敏试验,扩增其16SrRNA序列。结果显示,从病死猪肝脏中分离到2株革兰阴性杆菌,16SrRNA序列分析结果显示,1株菌与埃希菌属大肠埃希菌(登录号:KY367384.1)的同源性达到100%;另1株与变形杆菌属普通变形杆菌(登录号:EF426446.1)同源性达到99.9%。药敏试验结果显示,2株菌均对米诺环素较敏感,养殖户使用3d后,该猪场疫情即得到了控制。ln order to clarify the dead reason of pigs in a pig farm of Wuhe Anhui,the livers of dead pigswere used to isolate the pathogens,the biochemical test and drug sensitivity test,amplified 16 S rRNA se-quence,homology analysis were conducted. The results showed that two isolates of Gram negative bacilliwere isolated,a strain homology to Escherichia coli （accession No KY367384.1） was 100% ;another strainhomology to Bacillus proteus （accession No EF426446.1） reached 99.9%. The drug sensitivity test showedthat two strains were sensitive to minocycline,and three days later, the epidemic situation of the pig farmwas controlled.

关 键 词：大肠埃希菌 变形杆菌 药敏试验 同源性分析 猪 

分 类 号：S852.61[农业科学—基础兽医学]