奶牛临床型乳房炎MRSA新疆流行株耐药基因的检测及MLST分型研究  

Study on Antimicrobial Resistant Gene Detection and MLST Genotyping of MRSA Strains from Cow Mastitis in Xinjiang

作　　者：孟丹[1] 孟庆玲[1] 乔军[1] 彭叶龙 李重阳[1] 才学鹏[3] 陈创夫[1] 

机构地区：[1]石河子大学动物科技学院,新疆石河子832003 [2]阿克苏地区动物疫病控制诊断中心,新疆阿克苏843000 [3]中国农业科学院兰州兽医研究所,甘肃兰州730046

出　　处：《家畜生态学报》2018年第2期66-70,共5页Journal of Domestic Animal Ecology

基　　金：兵团中青年科技创新领军人才计划(2016BC001);国家十三五重点研发计划子课题(2016YFD0500900);自治区科研创新项目(XJGRI2016040)

摘　　要：为了解新疆地区奶牛临床型乳房炎MRSA流行株耐药表型、耐药基因分布及分子分型特征,采用纸片扩散法检测了临床分离的71株金黄色葡萄球菌的耐药表型,并对MRSA进行判定;应用多重PCR方法对MRSA流行株进行耐药基因的检测及MLST分型。结果显示,71株金黄色葡萄球菌中共检出13株MRSA,检出率为18.3%。13株MRSA流行株均检出mecA和linA耐药基因,检出率为100.0%;7株检出tetm耐药基因,检出率为53.8%;4株检出msrB耐药基因,检出率为30.8%;4株检出AacA-Da耐药基因,检出率为30.8%;MLST分型结果发现,13株MRSA可分为6种ST型,分别为ST188、ST9、ST63、ST2700、ST968、ST2373,其中ST9型流行较为广泛。本研究为新疆地区奶牛MRSA流行株的耐药特性及分子特征提供了理论基础,为兽医临床合理用药提供了科学依据。In order to know resistance characteristics, distribution of drug resistance genes and molecu- lar characteristics of the MRSA epidemic strains of dairy cow mastitis in Xinjiang, Methicillin-resistant Staphylococcus aureus （MRSA） was determined by disc diffusion method to detect the resistant properties of clinical isolated 71 strains staphylococcus aureus. Resistant gene detection and MLST genotyping were conducted with the use of multiplex PCR method. The results show that a total of 13 strains of MRSA have been detected among the 71 S. aureu isolates, with the detection rate of 18.3%; all 13 strains were detected owning mecA, linA gene, with the detection rate of 100%, 7 strains were detected owning tetrn gene, with the detection rate of 53.8%, 4 strains were detected owning msrB gene, with the detection rate of 30.8%, 4 strains were detected owning AacA-Da gene, with the detection rate of 30.8%, six ST types were detected in 13 strains of MRSA, ST188, ST9, ST63, ST2700, ST968, ST2373 respectively, ST9 had been popular widely among them. This study explored the correlation between phenotypes of drug re- sistance and drug resistance gene and molecular epidemiological characteristics, which provides both a foundation to understand the characteristics of genotyping, and a reliable theory basis for veterinary clinical medication.

关 键 词：耐甲氧西林金黄色葡萄球菌 耐药基因 MLST分型 

分 类 号：S811.5[农业科学—畜牧学]