声光可调-近红外漫反射光谱法快速评价黄芪药材质量  

Rapid Quality Evaluation of Astragali Radix by AOTF-NIR

作　　者：任绪华 苏婷 姜文月 李亚东 郑广晶 高陆 

机构地区：[1]长春中医药大学药学院,长春130117 [2]吉林省现代中药工程研究中心有限公司,长春130012 [3]吉林长白山药业集团股份有限公司,吉林白山134300

出　　处：《中国药房》2018年第2期168-171,共4页China Pharmacy

基　　金：国家中药标准化项目(No.ZYBZH-C-JL-26);吉林省科技发展计划项目(No.20150301009YY;YYZX201503)

摘　　要：目的:建立快速评价黄芪药材质量的方法。方法:采用烘干法测定黄芪药材样品水分含量,采用高效液相色谱(HPLC)-蒸发光散射检测法(ELSD)测定黄芪甲苷的含量,采用HPLC法测定毛蕊异黄酮葡萄糖苷的含量(均作为参考值)。采用声光可调-近红外漫反射光谱法结合偏最小二乘法建立预测黄芪药材中上述指标含量的定量模型(作为预测值)。根据参考值,采集60批药材样品,采用一阶导数联合平滑降噪法预处理光谱,水分、黄芪甲苷、毛蕊异黄酮葡萄糖苷含量预测的最佳波段分别为1100~2300、1080~2160、1170~2230 nm。结果:药材样品中水分、黄芪甲苷、毛蕊异黄酮葡萄糖苷的含量测定方法学考察符合要求。水分、黄芪甲苷、毛蕊异黄酮葡萄糖苷定量模型校正均方根偏差分别为0.1323、0.0066、0.0025,预测均方根偏差分别为0.2371、0.0163、0.0047,校正集内部交叉验证相关系数分别为0.9759、0.9533、0.9680;定量模型内部验证偏差分别为1.43%、1.90%、1.84%,外部验证偏差分别为1.73%、2.68%、2.71%。结论:该方法快速、准确、简便、无污染,可用于黄芪药材质量的快速评价。OBJECTIVE： To establish the method for rapid quality evaluation of Astragali Radix. METHODS： The moisture of medicinal material was determined by oven drying method; the content of astragaloside Ⅳ was determined by HPLC-ELSD; the content of isoflavone glucoside was determined by HPLC （as reference value）. The partial least squares （PLS） method combined with acousto-optic ruinable filter-NIDRS was adopted to build quantitative model of above indexes in Astragali Radix （as predict value）. According to reference value, 60 batches of sample were collected. The spectra pretreatment was conducted by first deriva- tive method combined with Savitzky golay. The optimal bands of moisture, astragaloside 1V and isoflavone glucoside were 1 100- 2 300 nm, 1 080-2 160 nm, 1 170-2 230 rim,respectively. RESULTS： The content determination of moisture, astragaloside Ⅳ and isoflavone glucoside in samples were all in line with methodology requirements. The corrected mean square root deviation of quanti- tative model for moisture, astragaloside IV, ealyeosin glucoside were 0.132 3, 0.006 6, 0.002 5, respectively; predicted mean square root deviation were 0.237 1, 0.016 3, 0.004 7; internal cross validation coefficient of correction set were 0.975 9, 0.953 3, 0.968 0; internal verification deviation of quantitative model were 1.43%, 1.90%, 1.84%; external verification deviation were 1.73 %, 2.68 %, 2.71%, respectively. CONCLUSIONS： The method is rapid, accurate, simple,pollution-free, and can be used for rapid quality evaluation of Astragali Radix.

关 键 词：近红外漫反射光谱法 声光可调 黄芪 水分 黄芪甲苷 毛蕊异黄酮葡萄糖苷 

分 类 号：R978.7[医药卫生—药品]