翻白草药材的HPLC指纹图谱及真伪鉴别研究  

Study on HPLC Fingerprints of Potentilla discolor and Authenticity Identification

作　　者：孔晓妮 许一平[2] 沈伟[2] 周洪雷[1] 

机构地区：[1]山东中医药大学药学院,济南250355 [2]山东中医药高等专科学校中药系,山东烟台264199

出　　处：《中国药房》2018年第2期172-175,共4页China Pharmacy

基　　金：山东省中医药科技发展计划项目(No.2013-141);山东省高等学校科技计划项目(No.J16LM54)

摘　　要：目的:建立翻白草药材的高效液相色谱(HPLC)指纹图谱,并进行真伪鉴别。方法:采用HPLC法,色谱柱为InertSustain C_(18),流动相为0.1%甲酸溶液-乙腈(梯度洗脱),流速为1.0 mL/min,检测波长为360 nm,柱温为30℃,进样量为10μL。以芦丁为参照,绘制19批翻白草药材样品和2批委陵菜药材样品的HPLC图谱,采用《中药色谱指纹图谱相似度评价系统》(2004)对21批药材样品进行相似度评价,确定19批翻白草药材样品的共有峰,采用SPSS 21.0统计软件进行聚类分析和主成分分析。结果:19批翻白草药材样品的HPLC图谱有18个共有峰,相似度均大于0.9,其HPLC图谱与对照指纹图谱具有较好的一致性;而2批委陵菜药材样品相似度均小于0.7。21批药材样品可聚为2大类,即2批委陵菜药材样品为一类,19批翻白草药材样品为一类,而翻白草药材样品可聚为4类;19批翻白草药材样品中芦丁、槲皮苷为主成分。结论:所建指纹图谱可为翻白草药材的真伪鉴别和质量评价提供参考。OBJECTIVE： To establish HPLC fingerprints of Potentilla discolor, and to conduct authenticity identification. METHODS： HPLC method was adopted. The determination was performed on InertSustain C18 column with mobile phase consisted of 0.1% formic acid solution-acetonitrile （gradient elution） at the flow rate of 1.0 mL/min, detection wavelength of 360 nm, column temperature of 30 ℃, sample size of 10 μL. Using rutin as reference, HPLC chromatograms of 19 batches of P discolor and 2 batches of P. chinesis were determined. TCM Fingerprint Similarity Evaluation System （2004） was used for similarity evaluation of 21 batches of samples, and common peak identification of 19 batches of P discolor. SPSS 21.0 statisticl software was used for main component analysis and cluster analysis. RESULTS： There were 18 common peaks in HPLC fingerprints of 19 batches of P discolor, the similarity was higher than 0.9. HPLC chromatogram was in good agreement with control fingerprint. The similarity of 2 batches of P chinesis was lower than 0.7. The 21 batches of medicinal materials could be grouped into 2 categories, 2 batches of P chinesis could be grouped into a category, 19 batches of P. discolor could be grouped into a category. P discolor could be grouped into 4 categories. Rutin and quercitrin were main ingredients in 19 batches of P. discolor. CONCLUSIONS： Established fingerprint can provide reference for authenticity identification and quality evaluation of P discolor.

关 键 词：翻白草 高效液相色谱法 指纹图谱 真伪鉴别 

分 类 号：R917[医药卫生—药物分析学]