MEKC测定麻花艽中齐墩果酸和熊果酸含量  

Determination of Oleanic Acid and Uosolic Acid in Gentiana straminea Maxim. by MEKC

作　　者：张军文 李成思 卢永昌[1,2,3] 史生辉 

机构地区：[1]青海民族大学药学院,青海西宁810007 [2]青海民族大学青海省青藏高原植物资源化学研究重点实验室,青海西宁810007 [3]青海民族大学青海省药物分析重点实验室,青海西宁810007

出　　处：《广西师范大学学报（自然科学版）》2018年第1期99-104,共6页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金(81160554)

摘　　要：用胶束毛细管电泳法(MEKC)对麻花艽中齐墩果酸和熊果酸的含量进行测定。以70 mmol/L硼砂—35 mmol/L十二烷基磺酸钠(SDS)—体积分数20%的乙腈(pH=9.85)为缓冲溶液,未涂渍标准熔融石英毛细管(有效长度为56cm,64.5cm×50μm)为分离通道,检测波长为210nm,分离电压为15kV,压力进样为5kPa×5s。结果显示2种活性成分的浓度与峰面积的线性关系良好(r>0.999 2),2种活性成分峰面积的RSD分别为3.21%、2.87%。该方法操作简单,精密度、重复性较好,可用于麻花艽的质量评价和控制。A micellar electrokinetic chromatography （MEKC） method was established for determining the contents of oleanic acid and ursolic acid in Gentiana straminea Maxim.. The separation was performed on an uncoated fused silica capillary （64.5 cm×50 μm,56 cm of effective length） with a voltage of 15 kV. A 20% acetonitrile solution containing 70 mmol/L borax and 35 mmol/L SDS（pH=9. 85） was used as the buffer solution. The sample was injected at a pressure of 5 kPa× 5 s and detected at a wavelength of 210 nm.The results showed a good linearity between the concentration and peak area （r 〉0.9992）. The RSD of two active ingredients were 4.21% and 3.87%. This MEKC method has the advantages of simple, accurate and reproducible. It could be used for the quality evaluation and control for Gentiana strarninea Maxim..

关 键 词：胶束毛细管电泳(MEKC) 麻花艽 齐墩果酸 熊果酸 含量 

分 类 号：O657.8[理学—分析化学] O622.5[理学—化学]