小鼠杏仁中央核逆向投射的研究  

Reverse Projection of the Central Nucleus of Amygdala in Mice

作　　者：谢静[1,2] 唐贺 林万华 孙华英[1] 吴桂生 和晓明[1] 邓科 罗怀容 

机构地区：[1]中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,云南昆明650201 [2]中国科学院大学生命科学院,北京100039 [3]河南省人民医院,河南郑州450003 [4]河南省眼科研究所,河南郑州450003 [5]广西师范大学生命科学学院,广西桂林541006

出　　处：《广西师范大学学报（自然科学版）》2018年第1期149-157,共9页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金(81671405);云南省自然科学基金(2012FB181);昆明医科大学应用基础研究联合专项(2017FE467-160);中国科学院昆明植物研究所国家重点实验室自主课题(P2015-ZZ17);国家自然科学青年基金(81703497)

摘　　要：杏仁核作为边缘系统的重要组成部分,与情绪异常的精神疾病相关,而杏仁中央核和基底核在恐惧条件化、动机奖赏、药物成瘾以及颞叶癫痫等疾病中起到重要作用。神经连接是神经元行使功能的生理学基础,近年来嗜神经病毒已经成为方便有效的神经环路示踪技术。本文采用一种新型的狂犬病毒RV,该病毒是一种嗜神经病毒,能在神经中逆向传导,且不跨突触。将该病毒注射到杏仁中央核,7d后取脑组织进行切片观察,发现病毒标记到杏仁核区域,如纹状体、杏仁核基底核、外侧核、前端杏仁核背侧、杏仁核纹状体过渡区,以及皮层各区域。此外还包括丘脑和下丘脑的某些区域。本文研究证明杏仁中央核接收来自很多脑区的广泛、直接的纤维输入,研究结果对于后续杏仁中央核的功能研究具有重要的参考价值。The amygdala is an important part of the limbic system that is associated with mental disorders. The central nucleus and basal nucleus of amygdala both play vital role in condition fear, motivational reward, drug addiction and temporal lobe epilepsy. The fiber connection is the physiological basis for their neural function. Nowadays,the neurotropic viruses have become an effective tool for the investigation of the neuroconncetion. A new type of rabies virus（RV）, being a reverse virus that cannot cross synapse, was used in this experiment, which ensures the specificity of the projection in our experimental results. The virus was iniected into the central nucleus of amygdala. After 7 days,the brain slice was observed and many brain areas were labeled, including striatum, basal nucleus of amygdala, lateral nucleus of amygdala,interstitial nucleus of the posterior limb of the anterior commissure medial part,anterior amygdaloid area dorsal part and other part of amygdala, cortical regions et al. Our results show that the central nucleus of amygdala receives a wide range of direct nerve fibers projections from many brain regions which may indicate physiological function of amygdala.

关 键 词：杏仁核中央核 逆向投射 RV病毒 嗜神经病毒 

分 类 号：Q422[生物学—神经生物学]