The rapid assembling of oligosaccharides by the developed HASP strategy  

作　　者：Yunhe Wang Shuai Meng Tingting Yue Shuchun Li Zhongjun Li 

机构地区：[1]State Key Laboratory of Natural and Biomimetic Drugs, Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University

出　　处：《Chinese Chemical Letters》2018年第1期69-72,共4页中国化学快报（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China(No. 21232002);the National Basic Research Program of China(No. 2012CB822100)

摘　　要：A new carrier containing three hydrophobic octadecyls was employed to improve the yield and purity of the synthesized oligosaccharides, and the applicability of hydrophobic carrier for HASP （hydrophobically assisted switching phase） was also explored. High performance liquid chromatography （HPLC） was used to analyze the purity of oligosaccharides and improve the elution efficiency. Elution system was optimized and replaced by isopropyl alcohol and water, in which the excess donor and other impurities had a better solubility than that in methanol.A new carrier containing three hydrophobic octadecyls was employed to improve the yield and purity of the synthesized oligosaccharides, and the applicability of hydrophobic carrier for HASP （hydrophobically assisted switching phase） was also explored. High performance liquid chromatography （HPLC） was used to analyze the purity of oligosaccharides and improve the elution efficiency. Elution system was optimized and replaced by isopropyl alcohol and water, in which the excess donor and other impurities had a better solubility than that in methanol.

关 键 词：Hydrophobically assisted switching phaseRapid oligosaccharide assemblyHPLCElution system 

分 类 号：O6[理学—化学]